通过EMS诱变普通小麦品系H261获得一个稳定遗传的斑点叶突变体LF2010。在自然条件下, 该突变体在三叶期叶片基部开始出现黄色斑点, 随后逐步扩散到全片叶、叶鞘、颖壳和麦芒。斑点部位不存在细胞死亡, 斑点性状的表达受光照和温度诱导, 突变体的色素含量、光合速率随着斑点的出现而显著下降。突变体的株高、有效穗数、单株产量、穗长、结实率和旗叶长等农艺性状显著下降, 但是千粒重和旗叶宽却与野生型无差异。将突变体与正常绿色品系杂交, 对其F1、F2和BC1代的遗传分析表明, LF2010的突变性状由1对隐性核基因控制。
A light and temperature affected spotted-leaf mutant LF2010 (Triticum aestivum L.) was obtained from a mutagenic treatment with EMS on a wheat line H261. The bright yellow spot became evident once the third leaf will expanded, then covered later leaves and leaf sheaths of the whole plant including tissues of spike. The mutant plants differed from normal plants in terms of total chlorophyll content and net photosynthetic rate once symptom appeared on the leaves. The plant height, spike length, spike number per plant, grain weight per plant, grain number per spike, seed setting rate, and flag leaf length were lower in the mutant than in the wild type. However, the 1000-grain weight and flag leaf width of the mutant had no significant difference with those of the wild type. Genetic analysis based on F1, F2, and BC1 populations revealed that the yellow spot trait was controlled by a single recessive gene.