中文摘要：

目的：分析结直肠癌MICA基因的表达与p53、K-ras基因突变的关系。方法：回顾性分析我院2014年3月至2016年3月收治的66例结直肠癌患者的临床资料,应用半定量PCR-SSCP技术,对癌组织mdr1基因表达量与p53、K-ras进行检测,并分析结直肠癌MICA基因表达与p53、K-ras基因突变间的相互关系。结果：经研究发现,MICA表达与结直肠癌患者肿瘤部位、年龄、性别无相关性（P〉0.05）。于66例患者中,22例淋巴结转移者的MICA平均表达量明显高于淋巴结未转移者,差异具有统计学意义（P〈0.05）。8例K-ras、p53基因联合突变者MICA基因平均表达量高于非联合突变者,差异具有显著统计学意义（P〈0.05）,12例K-ras基因突变者MICA平均表达量稍高于未突变者,但比较差异无统计学意义（P〉0.05）,16例p53基因突变者MICA平均表达量显著优于p53基因未突变者,差异具有统计学意义（P〈0.05）。结论：MICA基因高表达已成为了结直肠癌的关键检测指标,而p53、K-ras基因与其高表达具有相关性。

英文摘要：

Objective： To investigate the relationship between the expression of MICA gene in bladder cancer and the mutation of p53 gene and K-ras gene. Methods： 66 cases of bladder cancer patients with clinical data in our hospital from March 2014 to March 2016 were analyzed. Semi quantitative PCR-SSCP technique was used to detect the expression of MDR1 gene and p53 and K-ras in cancer tissues,and analyzed the relationship between the expression of MICA gene in colorectal cancer and the mutation of p53 and K-ras genes. Results： The study found that there was no correlation between the expression of MICA and the location,age and sex of the patients with colorectal cancer（ P〉0.05）. In 66 patients,the mean expression of MICA in 22 lymph node metastasis patients was higher than that without lymph node metastasis,and the difference was statistically significant（ P〈0.05）. 8 cases of K-ras and p53 gene expression of the MICA gene mutation combined with average weight is higher than non mutation,the difference was statistically significant（ P〈0.05）,12 cases of K-ras gene mutation MICA average expression was slightly higher than that without mutation,but the difference was not statistically significant（ P〉0.05）,16 cases of p53 gene mutation MICA expression was significantly better than the average mutation in the p53 gene,the difference was statistically significant（ P〈0.05）. Conclusion： The high expression of MICA gene has become a key indicator for the detection of bladder cancer,and the correlation between K-ras and p53 gene and its high expression.