生物预告的处理是克服从微生物由木质素降级酶劈开木质纤维素的 supermolecular 结构的 biorecalcitrance 的一个有希望的方法。有白腐烂真菌 Phanerochaete chrysosporium 的玉米 stover 的稳固的州的发酵被执行,这个预告的处理的效率被评估。当玉米 stover 是时,酶的水解作用收益到达了最大值如果稳固的州的发酵被执行超过九天,生物学上,为九天的 pretreated,和水解作用收益严厉地减少了。对这锋利的减少的可能的解释是那不仅降级的木质素酶(嘴唇和 MnP ) 被分泌,而且另外的代谢物,它对导致更低的水解作用收益的水解作用酶有毒或致命在 biopretreatment 的延长时期期间被产生。这些结果是有用的帮助决定最佳的预定并且在生物预告的处理过程理解木质素结构和降级机制。
Biological pretreatment is a promising way to overcome the biorecalcitrance of cleaving the super- molecular structure of lignocellulose by lignin degrading enzymes from microorganisms. Solid state fermentation of corn stover with the white-rot fungus Phanerochaete chrysosporium was carried out and the efficiency of this pretreatment was evaluated. The enzymatic hydrolysis yield reached a maximum when the corn stover was biologically pretreated for nine days, and the hydrolysis yield decreased sharply if the solid state fermentation was carried out for more than nine days. A possible explanation for this sharp decrease is that not only the lignin degrading enzymes (LiP and MnP) were secreted, but also other metabolites, which were toxic or fatal to the hydrolysis enzymes resulting in the lower hydrolysis yield were generated during the prolonged period of biopretreatment. These results are usefuI to help determine the optimal timing and to understand the lignin structure and degradation mechanism in biological pretreatment processes.