中文摘要：

目的探讨DNA结合抑制因子2（Id2）在大鼠脑室管膜前下区（SVZa）神经干细胞（NSCs）发育分化中的作用。方法运用RT-PCR、Western blotting、免疫组织化学技术，观察Id2在不同发育阶段SVZa-喙侧迁移流（RMS）-嗅脑（OB）流通道中的时空表达模式。结果从空间上看，Id2在RMS表达始终最高，SVZa次之，在OB表达最弱；从发育的时间来看，Id2在各区域胚胎时期表达均比刚出生时强；在OB和RMS区，成年比刚出生时表达强，老年仍有较强表达；在成年SVZa表达比刚出生时弱。结论Id2在SVZa神经干细胞的发育学表达模式提示，Id2可能有促进SVZa神经干细胞增殖和抑制其在RMS迁移区分化的作用。

英文摘要：

Objective To know the role of inhibitor of DNA binding 2（Id2） in the development of neural stem cells （NSCs） in the anterior subventricular zone（SVZa） . Methods To examine the express/on of Id2 in SVZa, rostral migratory stream （RMS） and olfactory bulb （OB） in E14, P0,P7, P14, P30 adult and old rat brains by reverse tnmscfiptase-pelymerase chain reacfion（RT-PCR） ,Western blotting and immunohistochemistry. Results The expression of Id2 peaked at the area of RMS, and was less positive in SVZa and the least in OB at different time courses of developing rat brains. The expression of Id2 in the three areas in El4 were higher than that on the postnatal day, higher in OB and RMS in adult than that on P0, and remain high in old. However, the expression of Id2 in SVZa in adult was weaker than that on P0. Conclusion The expression of Id2 in the deveolpment of SVZa NSCs indicates that Id2 plays an important role in blocking the differentiation of SVZa NSCs and increasing the proliferation of SVZa NSCs.