中文摘要：

采用微吸管技术，研究整合素α2β1对肝细胞癌（HCC）细胞SMMC-7721与Ⅳ型胶原衬表面粘附力特性和该细胞对Ⅳ型胶原趋化行为的影响．观察Anti-α2、Anti-β1对SMMC-7721细胞与Ⅳ型胶原裱衬表面的粘附力的影响；采用双微吸管实验法，观察SMMC-7721细胞伪足形成过程以及Anti-α2、Anti-β1对SMMC-7721细胞伪足形成的影响．利用流式细胞仪对SMMC-7721细胞表面整合素α2、β1亚单位的表达进行分析．结果表明，SMMC-7721细胞与5μg／mL Ⅳ型胶原裱衬表面之间的粘附力为952±134（×10^-10 N，n=60），加入5μg／mL Anti-α2粘附力减小到605±128（×10^-10 N，n=60）；加入10μg／mL Anti-α2时粘附力减小到579±57（×10^-10 N，n=50）．加入5μg／mL Anti-β1粘附力减小到449±119（×10^-10 N，n=60）；加入10μg／mL Anti-β1时粘附力减小到220±78（×10^-10 N，n=55）．双微吸管趋化实验表明：两侧微吸管加入相同浓度Ⅳ型胶原，细胞向两侧微吸管均有伪足形成；在此基础上，微吸管分别加入Anti-α2、Anti-β1的一侧，SMMC-7721细胞伪足生长曲线呈现明显的抑制，而未加入抗体的微吸管一侧与加入的对侧相比，该侧细胞的伪足明显增加．流式细胞仪分析表明，所研究细胞整合素α2、β1亚单位表达率达分别为23．17％和95．78％．上述结果表明，整合素α2β1是联系SMMC-7721肝癌细胞与Ⅳ型胶原裱衬表面粘附以及SMMC-7721细胞对Ⅳ型胶原趋化性伪足形成的重要受体基础．

英文摘要：

To quantitatively describe the effect of integrinα2β1 on the adhesion of hepatocellular carcinoma （HCC） cells （SMMC -7721） to type Ⅳ collagen （Col Ⅳ） coated surface and the chemotaxis of SMMC -7721 to Col Ⅳ. A micropipette technique was adopted to investigate the effect of blockade of integrinα2β1 on adhesion of SMMC -7721cells onto Col Ⅳ coated surfaces and pseudopod protrusion of SMMC -7721 cells in response to Col Ⅳ stimulation. Adhesion strength was expressed. Chemotactic pseudopod protrusion of a SMMC -7721 cell was evaluated using a dual - pipette set - up, in which two pipettes filled with Col Ⅳ solution were positional in close contact with the same cell. The lengths of pseudopods were measured and plotted against time to obtain a pseudopod growth curve. The integrinα2β1 on the surfaces of SMMC -7721 cells was analyzed with flow cytometry. The results show that the adhesion forces for SMMC - 7721 cells adhering on 5 μg/mL Col Ⅳ coated surfaces were 952±134（×10^-10 N, n =60）. Upon treatment of SMMC - 7721 cells with Anti - α2 in a protein concentration of 5 μg/ml and 10μg/mL , the value decreased to 605±128 （×10^-10 N, n = 60） and 579±57 （×10 ^-10 N, n = 50） respectively. In addition, upon treatment of SMMC - 7721 cells with Anti - β1 in concentration of 5 μg/ml and 10 μg/ml, the value decreased significantly to449±119（ × 10^-10 N, n = 60） and 220±78（ ×10^-10 N, n =55）, respectively. In dual pipette chemotaxis experiment, when the two pipettes were filled with Col Ⅳ, pseudopods extended from the SMMC -7721 cell into each of the pipettes nearly symmetrically, i. e. , with nearly identical maximum pseudopod length and similar pseudopod growth curves. Upon addition of Anti - α2 to one of the pipettes, pseudopod protrusion was blocked while protrusion into the opposite pipette became more evidently. In the same way, upon addition of Anti - β1 to one of the pipettes, pseudopod protrusion was also blocked nearly completely while protrusion