中文摘要：

通过一步反应合成了一种次氯酸（HOCl）荧光探针罗丹明6G酰肼，利用核磁和质谱对探针结构进行表征，并研究了其检测HOCl的荧光性能。实验结果表明，探针对HOCl的荧光分析具有高的灵敏性和选择性。利用不可逆的氧化还原反应，探针在中性溶液中快速产生与HOCl浓度呈线性比例的荧光增强信号，检测的线性范围为2—400μmol／L，检出限为0．06p,mol／L，其他常见活性氧物种对检测影响小。探针能检测自来水中HOCl的含量，并在HeLa活细胞中实现了HOCl的荧光成像。

英文摘要：

A fluorescent probe, rhodamine 6G hydrazide for hypochlorous acid （HOCI） was facilely synthesized by a one-step reaction, based on the rhodamine structure as the fluorophore and hydrazine as the recognition group. Its structure was characterized by nuclear magnetic resonance （NMR） and mass spectrometry（ MS）, and its fluorescence properties of HOCI detection were also investigated. The results indicated that the probe displayed a good sensitivity and selectivity to HOCI. Using an irreversible oxidation -reduction reaction, the probe showed a rapid fluorescence enhancement proportional to the amount of HOCl in neutral buffer with a linear range of 2 - 400 μmol/L and a detection limit of 0. 06 μmol/L, and other common reactive oxygen species（ROS） revealed relatively inactive interfences to the detection system. The probe was successfully applied in the determination of HOCI in tap water and fluorescence imaging of HOCl in living HeLa cells.