中文摘要：

为了建立适用于鼠尾草属植物的AFLP技术体系,并对鼠尾草属部分物种遗传多样性进行分析。作者经筛选得到24对有效AFLP引物组合,共检测1616个有效扩增位点;其中22对AFLP引物组合针对不同材料可检测到特异性扩增位点,每对引物检测强度从1到16个位点不等;依据AFLP数据计算的22个鼠尾草属植物材料间231个配对遗传相似系数介于0.5677～0.9898,显示了丰富的遗传多样性;系统树显示AFLP分析可以将11个鼠尾草属植物准确区分,其中种内不同居群的材料可有效聚为亚组,说明本文所构建的AFLP技术体系能够有效应用于鼠尾草属植物种间及种内鉴定、遗传多样性分析及系统发育研究中。

英文摘要：

To establish the AFLP fingerprinting system of good reproducibility and high distinguishability.The genetic diversity in the plants from the genus Salvia was analyzed by using AFLP.The 24 selected amplification primer combinations produced a total of 1616 amplified fragments and 1 to 16 special amplified fragments were obtained,according to different primer combinations used.The coefficient of gene similarity ranged from 0.5677 to 0.9898 between species.This wide distribution indicated that the genetic diversity of Salvia was plentiful.The cluster map including all samples was obtained by UPGMA method.Based on the results of AFLP,all samples could be classified into 11 clusters,which were consistent with the Salvia＇s taxonomy,and each sample could be correctly identified.The results indicated that the AFLP fingerprinting analysis system could be used effectively to identify the species,analyse the genetic diversity and system evolution of the genus Salvia.