中文摘要：

目的利用超高效液相色谱-飞行时间质谱（UPLC-TOF-MS）联用技术研究不同配伍比例的人参一附子的物质基础，并分析配伍比例对人参皂苷溶出变化的影响。方法采用Acquity HSS T3（100mm×2．1mm，1．8μn）色谱柱，乙腈一水系统（含0．1％甲酸）梯度洗脱，质谱仪检测，分析人参皂苷。以液相色谱图峰面积表示人参皂苷溶出量。结果人参与附子配伍后，大部分人参皂苷的溶出量随着人参比例下降呈线性下降；三醇型人参皂昔Re和齐墩果酸型人参皂苷Ro的溶出量比理论值增加；二醇型人参皂苷Rb2、Rb3和Rd及其丙二酸甲酰基衍生物的溶出量比理论值减少。结论人参．附子配伍后，各种人参皂苷的溶出现象不同：这些人参皂苷的溶出量变化可能与人参．附子配伍的药效有关。

英文摘要：

Objective Ultra performance liquid chromatography coupled with time-of-fight mass spectrometry （UPLC-TOF-MS） was used to reveal the chemical profile of Ginseng Radix and Aconiti Lateralis Radix Praeparata compatibility in different ratios, and to evaluate the content changes ofginsenosides. Methods The analyses were performed on a reversed-phase HSS T3 column （100 mm×2.1 mm, 1.8 μm） using a binary eluent （aqueous 0.1% formic acid, 0.1% formic acid-acetonitrile） under gradient conditions. Identifications of ginsenosides were confirmed by comparing MS and retention time with those of the reference compounds and the contents of ginsenosides were represented by the peak area of UPLC-TOF-MS chromatogram. Results After combined with Aconiti Lateralis Radix Praeparata, the contents of the most ginsenosides linearly decreased as the ratio of Ginseng Radix reduced. The dissolution of protopanaxatriol type ginsenoside Re and oleanolic acid ginsenoside Ro was promoted, protopanaxadiol type ginsenosides Rb2, Rb3, Rd and their malonyl derivatives exhibited the suppressed dissolution. Conclusion The dissolution of ginsenosides is diverse after the codecoction with Aconiti Lateralis Radix Praeparata, which may be related to the pharmaceutical effect of the compatibility of Ginseng Radix and Aconiti Lateralis Radix Praeparata.