中文摘要：

本试验以阿维菌素染毒原代培养的王鸽脑神经细胞为模型，观察阿维菌素对神经细胞凋亡的毒性作用和一氧化氮产生量的影响。采用原位末端标记法观察细胞凋亡，四唑盐比色法检测神经细胞相对存活率，流式细胞术检测凋亡细胞百分比，分光光度法测定细胞一氧化氮的产生量。结果显示，阿维菌素可诱导神经细胞凋亡和细胞坏死，并具有随着阿维菌素剂量递增而增加的趋势，同时阿维菌素也能引起一氧化氮产生量降低，但是未发现存在剂量效应关系。

英文摘要：

The effects of apoptosis and NO level were explored with model of AVM on king pigeon brain neurocytes in vitro culture. Apoptosis was observed by TUNEL, the relative survival rate of neurocytes was detected by MTT, the percentage of apoptotic cells was detected by Flow cy- tometry, and the production of NO in cells was acquired by absorption spectrometry. The results showed that AVM can induce apoptosis and cytonecrosis of neurocyte, and has increased with the dose of AVM. AVM can reduced the production of NO, too, but no dose-response relationship.