中文摘要：

目的研究颈动脉粥样硬化微栓子阳性是否与基质金属蛋白酶-9（MMP-9）基因启动子C-1562T的多态性有相关性，为脑血管疾病的早期预防、诊断及治疗提供帮助。方法对被确诊为缺血性脑血管病的患者行颈动脉超声检查，筛选出患颈动脉粥样硬化不稳定斑块的患者120例，再行彩色经颅多普勒超声（TCD）监测微栓子，将其划分为微栓子阳性组63例与微栓子阴性组57例，用聚合酶链反应-限制性片段长度多态性（PCR—RFLP）技术对MMP-9基因启动子C-1562T位点进行基因分型，并随机抽取10％的样本对MMP-9基因启动子C-1562T的基因型进行直接测序证实。统计2组患者中C-1562T的基因型、等位基因频率，并分析MMP-9基因启动子C-1562T的多态性是否与微栓子阳性有相关性。结果微栓子阳性组CT型和TT型明显高于微栓子阴性组，2组比较差异有统计学意义（#：32．568，P〈0．05）；MMP-9的T等位基因是微栓子形成的危险因素（OR：10．000，P〈0．05）。结论MMP-9基因启动子C-1562T基因多态性与微栓子形成密切相关。

英文摘要：

Objective To investigate the relationship between polymorphism at promoter of C - 1562 T of matrix metalloprotein- ases - 9 （ MMP - 9） and positive of carotid atherosclerosis micro embolus, and to provid the help for cerebrovascular disease prevention, early diagnosis and treatment. Methods A diagnosis of carotid ultrasound ischemie cerebrovascular were done for disease patients, and they were screened with 120 patients who had unstable plaques with carotid atherosclerosis. Further, color Transcranial Doppler ultra- sound （TCD） were used to monitor micro embolus and divided into the positive of micro embolus patients group （n = 63 ） and the nega- tive of micro embolus patients group （ n = 57）. At the same time application polymerase chain reaction - restriction fragment length poly- morphism （PCR - RFLP） technology was applied to type gene sites of MMP -9 polymorphism, and extract 10% the random samples to confirmed genotype of promoter C -1562T of MMP-9. Finally, using the statistics to compare the two groups genotype and allele fre- quency, and analyzes the promoter C - 1562 T of MMP -9 the polymorphism of whether there was a correlation with micro embolus posi- tive. Results CTtype and TY type were significantly higher in micro embolus positive group than these in the control group. There was statistical significance in the two groups （X2 = 32.568, P = 0. 000,P 〈 0.05 ）. The T allele of MMP- 9 was a risk factor of the forma- tion of micro embolus （ OR = 10. 000, P 〈 0.05 ）. Conclusion MMP - 9 gene promoter C - 1562 T sites intimately relate to the forma- tion of micro embolus.