中文摘要：

该研究以中间锦鸡儿（Caragana intermedia）为材料,利用RACE技术克隆了CiMYB68基因的全长序列。对CiMYB68的基因组DNA和cDNA全长分析显示,CiMYB68基因无内含子,开放阅读框为852 bp,编码284个氨基酸。预测CiMYB68基因编码的蛋白质等电点为8.95,分子量约为31 459.4 Da。序列比对和系统进化分析表明,该蛋白和大豆的GmMYB68一致性最高,达到67％。构建了CiMYB68基因与GFP融合表达质粒,激光共聚焦显微镜观察发现,融合蛋白定位于细胞核。用实时荧光定量PCR技术对在不同胁迫条件下CiMYB68基因的表达检测结果表明,在干旱和低温处理下CiMYB68均受到不同程度的诱导,暗示CiMYB68基因可能与中间锦鸡儿响应逆境胁迫有关。

英文摘要：

A MYB encoding gene was cloned by RACE（rapid amplification of cDNA ends） technique from Caragana intermedia.The gDNA and full-length cDNA sequence analysis revealed that this gene contains no intron.The full length ORF was 852 bp,and the deduced protein comprised 284 amino acids with a calculated molecular weight of 31 459.4 Da,as well as an isoelectric point of 8.95.Sequence alignment showed that this MYB protein is relatively close to GmMYB68,with an identity of 67%,so the gene was named as CiMYB68.CiMYB68 and GFP fusion vector was constructed,and GFP fluorescence was observed in the nuclei under confocal laser scanning microscope.Real-time quantitative PCR analysis showed that the transcript of CiMYB68 was induced strongly under drought and cold treatment.These results indicated that CiMYB68 might be involved in stress responses of C.intermedia.