中文摘要：

目的建立胆木药材及其制剂中原儿茶酸,短小舌根草苷,3-表短小舌根草苷,异长春花苷内酰胺的测定方法。方法采用HPLC法,X-SELECT CSHTMC18色谱柱（250 mm×4.6 mm,5μm）,流动相为乙腈-0.05%甲酸水,梯度洗脱;检测波长258 nm（原儿茶酸）,243 nm（短小舌根草苷、3-表短小舌根草苷）,226 nm（异长春花苷内酰胺）;体积流量1 mL/min;柱温30℃。结果原儿茶酸、短小舌根草苷、3-表短小舌根草苷和异长春花苷内酰胺分别在0.966～154.56μg/mL、0.944～151.04μg/mL、0.954～152.64μg/mL和0.916～146.56μg/mL内具有良好的线性关系（R2≥0.999 8）;4种成分在胆木药材中的平均加样回收率分别为98.2%、101.3%、97.4%和99.2%,RSD≤3.62%。在胆木注射液中的平均加样回收率为99.7%,103.0%,98.9%,100.7%,RSD≤2.44%。结论本法操作简便,结果可靠,重现性好,可作为胆木药材及其制剂质量控制的方法。

英文摘要：

AIM To develop a method for simultaneously determining protocatechuic acid,pumiloside,3-epipumiloside and strictosamide in Nauclea officinalis and its preparation—Danmu Injection.METHODS Chromatography was performed on an X-SELECT CSHTM C18 column（250 mm×4.6 mm,5 μm） with the mobile phase of acetonitrile and water containing 0.05% formic acid.The detection wavelengths were set at 258 nm for protocatechuic acid,243 nm for pumiloside and 3-epi-pumiloside,226 nm for strictosamide.The flow rate was 1 mL/min and column temperature was maintained at 30 ℃.RESULTS The correlation coefficients between concentrations and chromatographic peak areas of protocatechuic acid,pumiloside,3-epipumiloside and strictosamide were over 0.999 0 in the ranges of 0.966-154.56 μg/mL,0.944-151.04 μg/mL,0.954-152.64 μg/mL and 0.916-146.56 μg/mL,respectively.The average recoveries of the four compounds of Nauclea officinalis were 98.2%,101.3%,97.4% and 99.2%,respectively with RSD≤3.62%.The average recoveries of the four compounds of Danmu Injection were 99.7%,103.0%,98.9% and 100.7%,respectively with RSD≤2.44%.CONCLUSION The developed HPLC method is simple,accurate and has good reproducibility in separation,which is available for the quality control of Nauclea officinalis and Danmu Injection.