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中文摘要：
采用高效液相色谱／电喷雾飞行时间质谱联用技术（HPLC／ESI-TOF／MS），研究4种七叶皂苷的分子结构与裂解规律间的关系，并对娑罗子中的七叶皂苷类化合物进行鉴定。实验采用反相C18色谱柱，以乙腈-0．2％乙酸溶液为流动相，二元线性梯度洗脱，通过与电喷雾飞行时间质谱联用获得娑罗子中各皂苷成分的精确分子量和分子式；采用质谱碰撞诱导解离技术获得各化合物碎片裂解信息，结合文献对娑罗子中的14种皂苷类化合物进行了初步鉴定。研究表明，高效液相色谱-电喷雾飞行时间质谱联用技术是娑罗子中皂苷类化合物鉴别的有效工具。
英文摘要：
A convenient and user-friendly method based on high performance liquid chromatography （HPLC） and positive ion electrospray-time of flight-mass spectrometry （ESI-TOF/MS） has been developed for analysis and characterization of triterpene saponins in extract of Semen Aesculi. Samples were analyzed by a reversed-phase C18 column using a binary eluent of acetonitrile and water （with 0. 2% acetic acid） under gradient conditions. The [ M ＋ H]^＋ or [ M ＋ Na]^＋ ions of saponins in Semen Aesculi extract were observed by ESI-TOF/ MS on-line detection, and used to obtain accurate molecular weight and molecular formula information of saponins. Fourteen saponins in Semen Aesculi extract could be primary identified by accurate molecule weight information and fragmentation behavior obtained by collision induced dissociation experiment combined with literature review. This method has the advantages of simple operation, rapid measurement and accurate determination and it is a powerful tool for identification of saponins in Semen Aesculi.
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