中文摘要：

为提高酿酒废水产甲烷效率，采用新型单室无膜微生物电解池（MEC），以酿酒废水为基质，考察不同外加电压（0．4V、0．8V、1．2V）和传统消化（AD）对COD的去除、甲烷产生速率和能量回收的影响．结果表明，MEC外加电压为0．8V时，COD的去除负荷达7．09±0．74kg m3．d-1，较厌氧消化AD（4．19（±0．5）kg m-3 d-1）增加了69％．外加电压显著促进了乙醇的降解，0．4V、0．8V、1．2V的MEC乙醇降解速率分别为121．84．17±19．3mg L-1h-1、256．45±18．04mg L-1h-1、625．57±81．76mg L-1h-1，分别是AD（88．02±15．13mg L-1h-1）的1．38倍、2．91倍和7．1倍．外加0．8V，甲烷产生速率达到2019．78±76．41mL L-1 d-1，与AD（851．91±48．31mL L-1d-1）相比，增加了1．37倍；总能量回收率达到77．75％±0．88％，是AD（39．59％±2．31％）的1．97倍．循环伏安扫描（CV）发现MEC的碳毡在-0．270V附近和0．035V附近存在明显的还原峰和氧化峰．菌群高通量测序表明MEC的优势菌群为Methanothrix sp．和Geobacter sp．，其在混合菌群中的相对丰度分别为38．4％和12．83％，AD对应菌群的相对丰度仅为8．72％和1．21％．上述结果表明新型微生物电解池可显著促进酿酒废水的处理并提高甲烷产生速率和能量回收率．

英文摘要：

In order to increase the methane production rate of winery wastewater, this experiment adopted a new type of single-chamber microbial electrolysis cell （MEC） to treat winery wastewater. We investigated the removal efficiency of COD, methane production rate, substrate removal and energy recovery efficiency under different applied voltage （0.4 V, 0.8 V, 1.2 V） conditions. The results showed that when the applied voltage was 0.8 V, COD removal load reached 7.09 ± 0.74 kg m-3 d-1, which was 1.69 times of the AD reactor 4.19 ± 0.5 kg m3 d-1. With the increase of the applied voltage, the degradation rate of ethanol was accelerated. In AD group, the degradation rate of ethanol reached 88.02 ± 15.13 mg L-1 h-1. With applied voltage of 0.4 V, 0.8 V, and 1.2 V, the corresponding rate was 121.84.17 ± 19.3 mg L-1 h-1, 256.45 ± 18.04 mg L-1 h-1, and 625.57 ± 81.76 mg L-1 h-1, respectively, which was 1.38, 2.91 and 7.1 times of AD. When the applied voltage was 0.8 V, methane production rate and the total energy recovery rate was 2019.78 ± 76.41 mL L-1 d-1 and 77.75 ± 0.88%, respectively, which was 2.37 times and 1.97 times of the 851.91 ± 48.31 mL L-1 d-1 and 39.59 ± 2.31% for AD. Cyclic voltammetry （CV） showed a significant reduction peak and oxidation peak of MEC group in the vicinity of -0.27 V and 0.035 V. Analysis by high-throughput sequencing found that the dominant flora of MEC were Methanothrix sp. and Geobacter sp., which accounted for 38.4% and 12.83%, respectively, of the mixed bacteria. But the AD groups were only 8.72% and 1.21%. The new type of microbial electrolysis cell can increase the methane production rate and improve the enery recovery rate at the same time.