中文摘要：

目的评价依托咪酯对大鼠肺缺血再灌注损伤时NF-κB活性的影响。方法SPF级健康成年雄性SD大鼠60只，体重180～220 g，采用随机数字表法分为4组（n＝15）：假手术组（S组）、肺缺血再灌注组（I/R组）、依托咪酯组（E组）和脂肪乳组（F组）。采用开胸夹闭左肺门45 min，再灌注120 min的方法制备大鼠肺缺血再灌注损伤模型。于再灌注前3 min，I/R组和S组耳缘静脉注射生理盐水1 ml，E组和F组分别注射依托咪酯0.3 mg/kg和脂肪乳0.3 mg/kg（均用生理盐水稀释至1 ml）。于再灌注120 min时快速处死大鼠，取肺组织称重并计算湿重/干重（W/D）比值；采用HE染色法观察肺组织病理学结果；TUNEL法计数凋亡肺细胞，计算细胞凋亡指数（AI）；Western blot法检测肺组织细胞核蛋白NF-κB表达；ELISA法检测肺组织TNF-α的含量。结果与S组比较，I/R组肺组织W/D比值、AI和TNF-α含量升高，NF-κB表达上调（P〈0.05）；与I/R组比较，E组肺组织W/D比值、AI和TNF-α含量降低，NF-κB表达下调（P〈0.05），F组各指标差异无统计学意义（P〉0.05）。E组肺组织病理学损伤较I/R组减轻。结论依托咪酯减轻大鼠肺缺血再灌注损伤的机制与抑制NF-κB活性有关。

英文摘要：

Objective To evaluate the effect of etomidate on the activity of nuclear factor kappa B（NF-κB）during lung ischemia-reperfusion（I/R）injury in rats.Methods Sixty SPF healthy adult male Sprague-Dawley rats, weighing 180-220 g, were divided into 4 groups（n=15 each）using a random number table： sham operation group（group S）, lung I/R group（group I/R）, etomidate group（group E）and fat emulsion group（group F）. Lung I/R was induced by 45 min occlusion of the left hilum of the lung followed by 120 min reperfusion.At 3 min before reperfusion, normal saline 1 ml was injected via the auricular vein in I/R and S groups, and etomidate 0.3 mg/kg and fat emulsion 0.3 mg/kg（diluted to 1 ml in normal saline）were injected via the auricular vein in E and F groups, respectively.The rats were sacrificed at the end of reperfusion, and the lungs were removed for examination of the pathological changes（using haematoxylin and eosin staining）and for determination of the wet to dry weight ratio（W/D ratio）, cell apoptosis（by TUNEL）, expression of NF-κB in nucleoprotein（by Western blot）and content of tumor necrosis factor-alpha（TNF-α）in lung tissues（by enzyme-linked immunosorbent assay）. Apoptotic index（AI）was calculated.Results Compared with group S, the W/D ratio, AI and content of TNF-α in lung tissues were significantly increased, and the expression of NF-κB in nucleoprotein was up-regulated in group I/R（P〈0.05）. Compared with group I/R, the W/D ratio, AI and content of TNF-α in lung tissues were significantly decreased, and the expression of NF-κB in nucleoprotein was down-regulated in group E（P〈0.05）, and no significant change was found in the parameters mentioned above in group F（P〉0.05）. The pathological changes of the lung were significantly attenuated in group E as compared with group I/R.Conclusion The mechanism by which etomidate reduces lung I/R injury is related to inhibition of NF-κB activity in rats.