中文摘要：

[目的]观察解毒祛瘀滋阴方对MRL/lpr狼疮鼠巨噬细胞TLR4信号通路的影响。[方法]MRL/lpr狼疮鼠分为模型组、强的松组、解毒祛瘀滋阴方组（以下简称：中药组）以及强的松加中药组（以下简称：中西药结合组）,分别以生理盐水、强的松、解毒祛瘀滋阴方中药和强的松加解毒祛瘀滋阴方中药灌胃4周,收集肺、腹腔、脾脏巨噬细胞,RT-PCR检测相关基因的表达。[结果]经强的松治疗后狼疮鼠肺巨噬细胞TLR4 mRNA和脾巨噬细胞TLR4蛋白都显著升高（P〈0.05）,而中西药结合组则可以显著降低升高的脾巨噬细胞TLR4蛋白水平（P〈0.05）。强的松还可以显著降低肺、腹腔巨噬细胞MyD88、IFN-α、iNOS mRNA等TLR4下游分子的表达（P〈0.05）,而中西药结合组可以显著升高已经降低的肺巨噬细胞MyD88 m RNA的表达（P〈0.05）。[结论]MRL/lpr狼疮鼠应用糖皮质激素后,巨噬细胞TLR4信号通路发生改变,解毒祛瘀滋阴方中药可以降低糖皮质激素造成的TLR4蛋白的异常表达。

英文摘要：

[Objective] To observe the effect of Jiedu Quyu Ziyin decoction on TLR4 signaling pathway in macrophages of MRL/lpr lupus alice. [Method] MRL/lpr lupus mice were divided into four groups： model group, prednisone group, Jiedu Quyu Ziyin decoction group（hereinafter referred to as： Chinese medicine group） and prednisone plus Chinese medicine group （hereinafter referred to as： combination of Chinese and western medicine group）. The mice were gavaged with saline, prednisone, Jiedu Quyu Ziyin decoction and prednisone added Jiedu Quyu Ziyin decoction for 4 weeks. Macrophages of lung, peritoneal and spleen were collected and the expression of related genes was detected by RT-PCR. [Result] TLR4 mRNA in lung macrophages, and TLR4 protein in splenic macrophages increased significantly（P〈0.05） after the treatment of prednisone. The increased TLR4 protein in splenic macrophages was significantly decreased by combining with Jiedu Quyu Ziyin decoction（P〈0.05）. Prednisone can significantly reduce the TLR4 downstream molecules such as MyD88, IFN-α, iNOS mRNA expression in lung and peritoneal macrophages（P〈0.05）. The decreased MyD88 mRNA in lung macrophages was increased significantly by combining with Jiedu Quyu Ziyin decoction（P〈0.05）. [Conclusion] TLR4 signaling pathway is changed in macrophages after glucocorticoid administration in MRI/lpr lupus mice. Jiedu Quyu Ziyin decoction can reduce the abnormal glucocorticoid-induced TLR4 protein expression.