中文摘要：

以Ho2O3为反应物,采用水热法制备了纳米磷酸钬（n-Ho PO4）,并利用场发射扫描电子显微镜（SEM）、能谱分析（EDS）对其进行形貌表征和元素组成分析。将n-Ho PO4和血红蛋白（Hb）复合材料修饰于裸玻碳电极（GCE）表面构建生物传感器,实现了对H2O2的电化学检测。采用循环伏安（CV）和电化学交流阻抗（EIS）技术对修饰电极进行表征,结果表明,Hb/n-Ho PO4/GCE对H2O2的还原具有良好的电化学催化效果;n-Ho PO4具有良好的导电性和生物相容性,促进了Hb与工作电极间的直接电子转移。研究了不同p H值和电化学扫速对修饰电极响应电流的影响。在优化实验条件下,此生物传感器对H2O2在50-1000μmol/L范围内表现出良好的线性关系,相关线性系数R=0.999,检出限为17μmol/L（S/N=3）。此生物传感器具有检测范围宽、稳定性和重现性好、抗干扰能力强等优点,可用于实际样品的检测。

英文摘要：

The nanomaterial of n-Ho PO4 was synthesized via hydrothermal method with Ho2O3 as raw material,and its morphology and component were characterized by SEM and EDS.The composite material of n-Ho PO4 and hemoglobin（Hb） was dropped onto the surface of the glassy carbon electrode（GCE） so as to construct the biosensor for detection of hydrogen peroxide.The whole process was characterized by cyclic voltammetry（CV） and electrochemical impedance spectroscopy（EIS）.The results indicated that（Hb/nHo PO4/GCE） possessed good electrochemical catalytic effect towards H2O2 reduction.The n-Ho PO4 showed good electrical conductivity and could promote the direct electron transfer rate between Hb and GCE.The effect of different p H value and electrochemical scan rates on the response current of the modified electrode was studied.Under the optimal conditions,the biosensor exhibited a good linear detection range from 50 to1000 μmol/L（correlation coefficient R = 0.999） with the detection limit of 17 μmol/L（S/N = 3）.The biosensor showed wide detection range,good stability and reproducibility and strong anti-interference ability,and could be used for real sample detection.