中文摘要：

目的探讨青蒿琥酯（artesunate,ART）对口腔扁平苔藓（oral lichen planus,OLP）患者外周血转录因子T-bet/GATA-3失衡表达的调节作用。方法通过密度梯度离心法分离OLP患者外周血单个核细胞（peripheral blood mononuclear cells,PBMC）,分别用不同浓度（0、1、100μmol/L）的青蒿琥酯和5%的碳酸氢钠溶液作用于PBMC 24 h,逆转录聚合酶链反应（RT-PCR）测定法和免疫印迹法（Western blotting）检测OLP患者PBMC中T-bet和GATA-3 mRNA和蛋白的表达。结果 T-betmRNA和蛋白的表达在100μmol/L青蒿琥酯组（0.54±0.26、0.93±0.40）均低于对照组（1.35±0.63、1.47±0.43）（P值均〈0.01）,GATA-3 mRNA和蛋白的表达在1μmol/L（0.62±0.33、1.11±0.69）和100μmol/L（0.23±0.21、0.44±0.27）青蒿琥酯组中均低于对照组（1.40±0.66、1.68±0.61）（P值均〈0.01）。结论青蒿琥酯能够调节OLP患者T-bet和GATA-3的失衡表达。

英文摘要：

Objective To investigate the effects of artesunate on expression of the T cell-specific transcription factors T-bet/GATA-3 in oral lichen planus（OLP）.Methods Peripheral blood mononuclear cells（PBMC） were isolated by density gradient centrifugation from OLP patients.The cells were treated with different concentrations of artesunate（0,1,100 μmol/L） and 5% sodium bicarbonate for 24 hours.Expression of T-bet and GATA-3 mRNA and protein in the PBMCs was detected by reverse transcription-polymerase chain reaction（RT-PCR） and western blotting respectively.Results The expression of T-bet mRNA and protein in 100μmol/L artesunate（0.54±0.26、0.93±0.40）was weaker than that in the control group（1.35 ±0.63,1.47±0.43）（P〈0.01,P〈0.01）.The expression of GATA-3 mRNA and protein in 1μmol/L（0.62±0.33,1.11±0.69） and 100 μmol/L（0.23±0.21,0.44±0.27）artesunate was weaker significantly than that in the control group（1.40±0.66,1.68±0.61）（P〈0.01,P〈0.01,P〈0.01,P〈0.01）.Conclusions Artesunate can rectificate the imbalance of transcription factors T-bet and GATA-3.Artesunate may be an option in the treatment of OLP.