目的研究国内食蟹猴种群的遗传背景特性,建立食蟹猴种群遗传质量监测方法。方法采用微卫星DNA遗传标记技术对50只食蟹猴种群个体进行遗传质量监测及DNA多态性分析。结果从100个微卫星DNA位点中筛选出20个多态性高的位点,其食蟹猴种群个体的等位基因数目为5-10条,个体间均呈现高度的多态性;其观察等位基因数(Na)为5.0~10.0,有效等位基因数(Ne)为4.6118~8.3404,基因多样性(H)为0.7832~0.8801和香隆信息指数(I)为1.5651~2.1592。结论本实验有效地分析了食蟹猴种群的遗传多态性,为今后筛选特异性微卫星位点来建立食蟹猴种群遗传质量监测方法提供了理论依据。
Objective To investigate the genetic background features of Macaca fascicularis and to establish the genetic quality monitoring method in Macaca fascicularis.Methods Using microsatellite DNA genetic markers,genetic quality monitoring and DNA polymorphism analysis were conducted in 50 Macaca fascicularis.Results 20 loci with high polymorphism was selected from 100 microsatellite DNA loci,among which allele numbers in Macaca fascicularis were 5-10,and individuals showed a high degree of polymorphism.Observed number of alleles(Na)was 5.0~10.0,effective number of alleles(Ne)was 4.6118~8.3404,gene diversity(H)was 0.7832~0.8801 and Shannon's information index(I)was 1.5651~2.1592.Conclusions The experiments effectively analyzed the genetic diversity of Macaca fascicularis,and it can provide a theoretical foundation for establishing genetic quality monitoring method with specific microsatellite loci selected in Macaca fascicularis.