中文摘要：

为获得具有细胞周期后期促进复合物（APC/C）活性的细胞裂解液并建立APC/C体外活性检测体系,以其底物Cyc-lin B及Securin的降解为检测指标,分别从细胞阻滞于有丝分裂期的时间,UBCH10（APC/C的E2酶）的浓度以及裂解液浓度等几个方面研究了各种处理因素对APC/C活性的影响,并且用体外翻译底物验证了其活性及相关结论。此项工作为进一步探索APC/C相关分子事件奠定了基础。

英文摘要：

To prepare the cell extracts with the APC/C activity and analysis of its influencing factors,the degradation of APC/C＇s substrates Cyclin B and Securin was used as a marker.Several assays were done to analysis its influencing factors,including the duration of cells arrest in mitosis,the activity of extracts,the presence of its E2 enzyme,UBCH10,and the concentration of extracts.The similar result was obtained using the in vitro translated substrates.Taken together,these assays were the basement to further study the related molecular mechanism.