应用MicroResp方法研究4种重建森林土壤微生物对不同类型碳源的代谢特征,自然恢复地为对照处理。结果表明,不同培养时间(6 h和继续培养18 h)和不同土壤深度(0~10 和10~20 cm),土壤微生物对单一碳源的利用能力差异显著。4种重建森林土壤微生物对碳源利用能力有显著差异,表现为:自然恢复地 〉 阔叶混交林 〉 阔叶纯林 〉 针阔混交林 〉 针叶纯林。多样性指数和均匀度指数表明4种重建森林土壤微生物群落多样性的变化趋势为:自然恢复地 〉 阔叶混交林 〉 阔叶纯林 〉 针阔混交林 〉 针叶纯林,除自然恢复地外,土壤微生物的多样性和均匀度在4种不同重建森林类型之间差异不显著。主成分分析结果表明,所选15种碳源能够阐述4种不同重建森林土壤微生物功能多样性的差异,森林土壤微生物的敏感碳源主要为糖类。
To investigate soil microbe using of different types of carbon sources under four types of reestablished forests or treatments (mixed broadleaf plantation, pure broadleaf plantation, mixed coniferous-broadleaf plantation, pure coniferous plantation) with a naturally restored fallow land as control, the microResp method was used. Results show that the capacity of soil microbes using mono-carbon source varied significantly with duration of incubation (6 h or additional 18 h) and soil depth (0-10 or 10-20 cm), and also with the type of forest. In terms of the capacity, the forests followed the order of naturally restored fallow land 〉 mixed broadleaf plantation 〉 pure broadleaf plantation 〉 mixed coniferous-broadleaf plantation 〉 pure coniferous plantation. Shannon index (H) and evenness index (E) indicates that the five treatments followed an order of mixed broadleaf plantation 〉 pure broadleaf plantation 〉 mixed coniferous-broadleaf plantation 〉 pure coniferous plantation in soil microbial community diversity. All the treatments, except the control did not differ much from each other in soil microbial diversity and abundance. Principle components analysis indicates that the fifteen carbon sources selected in this study were sufficient to elucidate differences between the four plantations in soil microbial functional diversity, and that sugars were the sensitive carbon source to forest soil microbes.