中文摘要：

花粉水和是在耻辱上决定花粉萌芽的一个关键步骤。家族是 SNF1related 蛋白质 kinase 的一个 plantspecific 子单元 1 建筑群(SnRK1 建筑群) 。在 Arabidopsis 家族异种的花粉，反应的氧种类的层次被减少它在耻辱上导致变异的花粉的损害水和。在这研究，我们由 RNAseq 在家族异种花粉分析了基因表示并且发现里面的 shaker K ⁺ 隧道 SPIK 的表示是在家族花粉的 downregulated。而且，我们证明 Arabidopsis spik 异种的花粉水和在 wildtype 耻辱上是有缺点的，尽管变异的花粉在 vitro 表明了正常水和。另外， spik 异种花粉的有缺点的水和不能被 wildtype 花粉在耻辱上救，显示 spik 变化剥夺了能力耻辱上的花粉吸收。我们的结果建议 Arabidopsis SnRK1 建筑群调整 SPIK 表示，它在在耻辱上决定花粉水和工作。

英文摘要：

Pollen hydration is a critical step that determines pollen germination on the stigma. KINγ is a plantspecific subunit of the SNF-1-related protein kinase 1 complex （SnRK1 complex）. In pollen of the Arabidopsis kinβγ mutant, the levels of reactive oxygen species were decreased which lead to compromised hydration of the mutant pollen on the stigma. In this study, we analyzed gene expression in kinβγ mutant pollen by RNA-seq and found the expression of inward shaker K^＋ channel SPIK was down-regulated in the kinβγ /pollen. Furthermore, we showed that the pollen hydration of the Arabidopsis spik mutant was defective on the wild-type stigma, althoughthe mutant pollen demonstrated normal hydration in vitro. Additionally, the defective hydration of spik mutant pollen could not be rescued by the wild-type pollen on the stigma, indicating that the spik mutation deprived the capability of pollen absorption on the stigma. Our results suggest that the Arabidopsis SnRK1 complex regulates SPIK expression, which functions in determining pollen hydration on the stigma.