中文摘要：

针对病鳗内脏中分离的5株致病性气单胞菌,采用琼脂稀释法测定五倍子、石榴皮、大黄、虎杖、黄芩及黄连各味中药的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）;再根据棋盘法设计15种双联用药方和4种三联用药方,同样检测各组合配方的抑菌作用。实验结果表明,6味中药对养殖鳗鲡5株致病性气单胞菌均有不同程度的抗菌效果,其中五倍子的抑菌作用最强,其次是大黄和石榴皮,而黄连的抑菌效果最差;15种双联用药方较各味中药单用的抑菌活性绝大多数出现增强,抑菌浓度至少减低39%,FIC〈1呈现协同效应的比例占85.7%,其中FIC≤0.5表现显著增强抗菌活性的协同比例占23.3%;4种三联用药方对5株致病性气单胞菌均具有显著的协同抑制效应,复方中单味中药的抑菌浓度可以降低80%以上;而双联用HC14对4株致病菌出现FIC≥2的降低彼此抗菌活性的相互拮抗现象。由此说明合理运用不同中药的联用配伍,不仅可提高单味中药的抗菌疗效,而且大大减少了单一中药在实际养殖生产中的给药浓度,降低药物在环境中的残留量,防止残留药物造成环境污染,并且降低用药成本,提高水产养殖业的经济和社会效益。研究为中药复方防治细菌性鱼病提供科学理论参考。

英文摘要：

Rhus chinensis Mill.,Rheum palmatum Linn.,Rhizoma polygoni Cuspidati,Punica granatum Linn.,Scutellaria Baicalensis georgi and Coptis chinensis Franch,were first ground into superfine powder respectively.Then they were mixed one by one based on chessboard experimental method to get nineteen kinds of compounds of Chinese herbs.To study antibacterial activity of the compounds,the minimum inhibitory concentration（MIC） and the minimum bactericidal concentration（MBC） of single drug and the compounds against five strains of pathogenic Aeromonas from eels were measured with agar dilution method.The results showed that six kinds of Chinese herbs had antibacterial effect at certain extent on the five bacteria.RCM had the best antibacterial activity,followed by PGL and RPL,CCF had the worst antibacterial activity.Fifteen double combinations had synergism antibacterial effects against pathogenic bacteria at different extend,and antibacterial activity of almost all compounds increased,whose inhibitory concentration reduced at least 39%.The ratios of FIC≤1 and FIC≤0.5 to all FIC were 85.7% and 23.3% respectively.Four kinds of triple combinations had synergism antibacterial effects against five strains of pathogenic Aeromonas significant synergistic inhibitory effect,whose inhibitory concentration reduced at least 80%.But FIC of prescription HC14 on the four pathogens was greater than two,which occurred the phenomenon of mutual antagonism,and resulted in antimicrobial activity reduce.The study also indicated that the rational use of Chinese herbal compounds could not only increase drug antimicrobial efficacy,but also could significantly reduce the concentration of Chinese herbs in the compounds.Therefore,the environmental pollution and the resistance to drug caused by medicaments also could be reduced greatly.Obviously,the results obtained from this study can lay a theoretical basis for exploitation of efficient,lower residual,safe and environmental friendly drugs against pathogenic bacteria from fishes.