中文摘要：

利用自行设计的生物膜培养装置进行挂膜,通过向生物膜反应器中投加不同浓度的Cu2＋,探讨生物膜上的胞外聚合物（extracellular polymeric substance,EPS）与生物膜去除金属铜之间的关系。研究结果表明：生物膜法较活性污泥法对Cu2＋具有更好的耐受性;当Cu2＋〈2 mg.L^-1,Cu2＋会抑制生物膜分泌EPS,在2 mg.L^-15 mg.L^-1,生物膜系统表现为严重的不稳定性;比较蛋白质（proteins,PN）/多糖（polysaccharide,PS）值发现,Cu2＋对生物膜的抑制主要是对生物膜上胞外多糖的抑制,而生物膜对Cu2＋的抵抗表现为分泌更多的胞外蛋白;EPS与Cu2＋的富集率关系呈线性正相关。

英文摘要：

Through adding different concentration of Cue＋ in the biofilm reactor, the relationship between extracellular polymeric substance（EPS） secreted by biofilm and efficiency of copper removal by biofilm was studied. Biofilm tolerance of Cu^2＋ was better than activated sludge, but a high concentration of Cu^2＋ rapidly poisoned the biofilm. When Cu^2＋〈2 mg.L^-1, Cu^2＋ inhibited biofilm secretion of EPS. Concentration of 2 mg＇L-1 〈Cu^2＋〈5 mg＇L-a, Cu^2＋ would increase the amount of EPS, while Cu^2＋〉5 mg.L-1, the biofilm system displayed serious instability. Comparing proteins/polysaccharide （PN/PS） value showed that the biofilm suffered inhibition by Cu^2＋. The biofilm would inhibit more extracellular polysaccharide secretion, and the biofilm resistance of Cu^2＋ indicated that the biofilm would secrete more extracellular protein. The amount of EPS had a good correlation with the enrichment ratio of Cu^2＋.