中文摘要：

目前，治疗葡萄酒色斑（PWS）等血管性皮肤病主要使用激光疗法，但治疗过程中血管的损伤机理仍不清楚，深入研究激光照射过程中血管形态的动态变化规律具有重要意义。自制了鼠脊视窗，利用CCD相机以及显微镜可视化观测Nd：YAG（1064nm）激光照射后血管形态的动态变化，并连续观测血管直径随时间的动态变化规律。实验结果表明，在单脉冲较低能量激光照射下，血管随激光能量的增加持续膨胀，能量累积到一定值时，血管出现收缩。多脉冲激光照射血管时，血管出现膨胀、血液凝固以及血管收缩等现象。有关激光照射后血管形态动态变化的规律，为今后更好地使用近红外长波激光治疗PWS提供了参考。

英文摘要：

Recently, laser therapy has been widely used for treating portwine stain （PWS） and other vascular lesions. However, the mechanism of the vascular injury during the treatment process is still not clear. In this paper, a hamster dorsal window chamber model is constructed to investigate the dynamic change of the vessel during pulsed laser treatment. A CCD camera and microscope are used to capture the dynamic variation of microvessels after 1064 nm laser pulse exposures. Experimental results show that the vessel continues expansion with the increasing of the radiant exposure, and vasoconstriction appears when the energy accumulates to a certain value with a single laser pulse. After multiple laser pulse irradiation, vessel expansion, blood coagulation and vasoconstriction are observed. These results may provide useful feedback for the treatment of PWS by near-infrared long wave laser.