中文摘要：

针对近年广泛应用于药代筛选的底物消除法，基于药物多衰减曲线，建立了酶促反应的线性化处理方程，提出了线性转换求解酶动力学参数的新方法。测定了9个探针底物在人肝微粒体中代谢的多衰减曲线，通过线性回归同时获得米氏常数Km、最大反应速率Vmax和内在清除率CLiat等参数，并与传统的底物消除曲线拟合法进行比较分析，验证了新方法的有效性。多衰减曲线线性作图是一种简便、直观、可靠的数据可视化处理方法。

英文摘要：

Aiming at the substrate depletion method widely used in multiple drug filtering, establishes a linear equation of enzymatic reaction based on drug decay curve and proposes a new method for linear conversion solution of enzymes kinetic parameters. Determines decay curves of nine probe metabolic reactions in human liver microsomes and obtains by linear regression the Michaelis constant K, maximum velocity of the metabolic reaction Vmax and intrinsic clearance CLint. Comparing with traditional substrate depletion curvilinear fitting method, verifies the effectiveness of the new method. Multi-linear decay curve mapping is a simple, intuitive and reliable data visualization method.