【目的】揭示叶片营养与种仁营养积累之间的关系,为薄壳山核桃果实营养积累、品质形成机制的研究及大小年现象等相关问题的解决提供理论依据。【方法】以薄壳山核桃‘波尼’品种为试材,连续2 a对其结果期全过程的果实和叶片进行动态取样,测定种仁和叶片中营养物质含量的变化情况,测定指标包括脂肪、可溶性糖、蛋白质、纤维素、水分及P、K、Ca、Mg、Na、Mn、Fe、Cu、Zn、Se等的含量。同时,对种仁及叶片中营养物质的变化进行相关性分析。【结果】薄壳山核桃种仁2 a的脂肪含量均呈现先上升后下降的趋势,可溶性糖、蛋白质、纤维素、含水量均呈下降趋势,种仁矿质元素在95~125 d表现出剧烈变化,而叶中各项指标变化相对较平缓。【结论】果实发育早期至中后期(95~145 d)是薄壳山核桃营养物质变化最明显的阶段,是果实品质积累的关键时期。
【Objective】The pecan, Carya illinoinensis(Wangenh.) K. Koch, is the most economically important member of the Carya Nutt of Juglandaceae.‘Pawnee’, originated from north American, has become the largest cultivar in both north American and China recently for its good fruit quality and disease resistance, but the dynamic changes in nutrients in‘Pawnee’have not been reported. Studies on dynamic changes in nutrients can help to understand the mechanism of nut quality formation, which is crucial for better use of this nut crop or breeding programs. Pecan has the serious problem of irregular bearing, which is most likely caused by unstable accumulation and transformation of nutrients. Analyzing the dynamic changes in nutrients in both the kernel and the leaf during nut development may obtain information of nutrient accumulation and transformation. In this study, dynamic changes in the nutrient contents both in the kernel and in the leaves and their correlations were examined in relation to quality formation and productivity in pecan‘Pawnee’.【Methods】Samples were collected from six healthy‘Pawnee’trees(8- 12 years old, planting space 5.0 m×7.0 m) with similar trunk diameter and tree vigor at the orchard of Nanjing Lvzhou Pecan Technology Co. Ltd.(latitude 31°14′-32°36′N; longitude 118°22′-119°14′E) located at Liuhe district, Nanjing, Jiangsu province, China. Fruit and leaf samples were collected every 10 daysfrom 95 d after full blossom during the fruiting period for two successive years. The contents of fat, solublesugars, proteins, fiber, moisture and mineral elements such as P, K, Ca, Mg, Na, Mn, Fe, Cu, Zn and Se in‘Pawnee’were measured and their correlations were analyzed. Fat content was determined by Soxhlet ex-traction method with the extraction solvent of hexane; protein concentration was measured by the Kjeldahlmethod; soluble sugar content was analyzed by anthrone colorimetry; fiber content was measured by nitricacid-ethanol method; and moisture content was