中文摘要：

目的：观察骨髓间充质干细胞（BMSCs）对糖尿病小鼠（db/db）创面中性粒细胞（PMNs）和巨噬细胞（MΦ）浸润及巨噬细胞炎性蛋白-2（MIP-2）和单核细胞趋化蛋白-1（MCP-1）mRNA 表达的影响。方法：40只C57BLKS/Nju背景自发突变糖尿病雄性小鼠（db/db小鼠）随机分为BMSCs治疗组和对照组（n=20）,均于背部制作2个0.8 cm×0.8 cm全层皮肤缺损创面模型。BMSCs治疗组于伤后当天（第0天）向全层皮肤缺损创缘皮下注射BMSCs 1×106个,对照组同法注射等体积PBS。IPP6.0图像分析软件测算创面形成后第1、3、5、7、10、14天创面愈合率；免疫组化染色后采用 IPP6.0病理图像分析软件测定吸光度值检测伤后第1、3、5、7、10天创面PMNs和第3、5、7、10、14天 MΦ浸润情况；用 qRT-PCR 相对定量法检测第1、3、5、7天创面组织中趋化因子MIP-2和 MCP-1 mRNA的表达。结果：BMSCs 治疗组和对照组的创面愈合时间分别为（11.67±0.58）d 和（16.33±0.58）d（P〈0.05）。BMSCs治疗组PMNs和 MΦ浸润高峰均较对照组提前,且高峰值增高（P〈0.01）；BMSCs组 MIP-2 mRNA表达高峰在创伤后第1天,为对照组30.91倍（P〈0.05）,MCP-1 mRNA表达高峰在创伤后第3天,为对照组96.88倍（P〈0.05）。结论：BMSCs 可能通过提高创伤部位趋化因子 MIP-2和 MCP-1表达进而促进早期炎症细胞P MN s和 MΦ浸润,改善糖尿病创面愈合。

英文摘要：

Objective：To study the effect of bone marrow mesenchymal stem cells （BMSCs）on infiltration of neutrophils （PMNs）and macrophages （MΦ）,mRNA expression of macrophage inflammatory protein-2 （MIP-2）,and monocyte chemo-tactic protein-1 （MCP-1）in a genetical modification-induced spontaneous diabetic murine model. Methods：Forty C57BLKS/Nj u spontaneous mutation resulted diabetic mice （db/db mice）were randomly divided into two groups：BMSCs group and control group （n=20）.Full-thickness skin defects of 8 mm×8 mm were created on the dorsum of each mouse. Mice in BM-SCs group were treated with 1×106 BMSCs via subcutaneous injection around the defect after injury,but in control group, PBS was injected instead of BMSCs. Wound healing rate was assayed on postwound day 1,3,5,7,10 and 14 with image pro-cessing software IPP6.0. The infiltration of PMNs （postwound day 1,3,5,7 and 10）and MΦ（postwound day 3,5,7,10 and 14）was observed by immunohistochemical method and optical density value detection. The relative quantitative mRNA expression of MIP-2 and MCP-1 of the wound tissues on postwound day 1,3,5 and 7 was determined by qRT-PCR relative quantitative analysis.Results：The wound healing time was （11.67±0.58）days and （16.33±0.58）days respectively in BM-SCs group and PBS group （P〈0.05）.Infiltration of PMNs and MΦwas more marked in BMSCs group（P〈0.01）,with earlier peaking than in PBS group. mRNA expression of MIP-2 and MCP-1 in BMSCs group was statistically elevated in postwound day 1 and day 3,respectively （P〈0.05 ）,which were 30.91 and 96.88 times of those in the control group.Conclusions：BMSCs might enhance infiltration of PMNs and MΦby promoting the expression of MIP-2 and MCP-1 in wound,and they might also contribute to accelerate healing process of diabetic wound when treated with BMSCs.