中文摘要：

从油井采出水中分离到一株高温产胞外聚合物的细菌MS-1，经16SrDNA基因序列分析属于芽孢杆菌属（Bacillus sp．）。该菌能在60℃生长并产生胞外聚合物，其中胞外多糖含量为48．3％-54．5％，主要由甘露糖、葡萄糖、半乳糖组成，摩尔比分别是2．04：1．00：0．89。胞外聚合物中蛋白含量为37．2％-42．4％，主要由甲硫氨酸、亮氨酸、天门冬氨酸、丙氨酸、组氨酸和丝氨酸组成。利用透射电镜和环境扫描电镜对胞外聚合物的形成进行了观察。该菌的分离和研究为高温油藏的微生物调剖和驱油奠定了生物学基础。

英文摘要：

A thermophilic bacterial strain MS-1 was isolated from the water let from an oil well, and identified as Bacillus sp. by its 16S rDNA sequence and morphological characteristics. The bacterium was able to grow at 60℃ and produce extracellular polymeric substance （EPS）. Analysis of the EPS showed that there was 48.3%-54.5% polysaccharide, which was composed of mannose：glucose：galactose at a ratio of 2.04 ：1.00 ： 0.89. The protein fraction was 37.2%-42.4% in EPS, containing methionine, leucine, aspartic acid, alanine, histidine and serine.Transmission Electron Microscopy and Environmental Scanning Electron Microscopy were used to observe the extracellular polymeric substances. The strain is useful in microbial profile modification in high temperature oil fields.