喜树(Camptotheca acuminata)是我国特有濒危植物,所含的喜树碱(camptothecin,CPT)是具有抗癌活性的单萜吲哚生物碱。喜树在我国南方已有大面积的人工种植,生产中存在喜树碱含量偏低等问题,这些问题阻碍喜树产业的健康发展。本文通过生理和化学调控的方法,研究了采后处理方式对喜树碱类化合物含量的影响。采后处理对叶片和果实的喜树碱含量有一定的影响。800 mg/L多效唑+25℃+紫外照射+剪碎的组合处理时,叶的喜树碱含量最高,达0.128%,是对照的1.8倍;100 mg/L马来酸+25℃+紫外照射+剪碎的组合处理时,果实喜树碱含量最高,达0.119%,是对照的1.3倍。在喜树采后采用生理调控是提高喜树碱类化合物含量的有效手段。
Camptotheca acuminat is a deciduous and endangered medicinal tree that generates the alkaloid camptothecin (CPT). which has anticancer effects. Camptothecin (CPT) is a monoterpene-derived indole alkaloid. Camptotheca acuminata has been planted widely in South China.The problems of low concentration of camptothecin impede the development of Camptotheca industry. In this paper, the physical control and chemical control methods were studied, mainly including the effects of post-harvest handling on the content of camptothecin derivatives.Integrated post-harvest treatments in the leaves and fruit improve the content of camptothecin to some degrees. Treatment of 800 mg/L PBZ, 25 ℃ of temperature, UV radiation and cutting can lead the content of camptothecin to 1.8-fold campared with control in leaves; 100 mg/L maleic acid, 25 ℃ of temperature, UV radiation and cutting can improve camptothecin content 1.3-fold campared with control in fruits. Physiological regulation in the course of Camptotheca post-harvest is effective means to promote the content ofcamptothecin compounds.