中文摘要：

为了解翘嘴鳜mi R-222的时空表达规律,研究利用实时荧光定量PCR的方法检测mi R-222在翘嘴鳜不同组织、胚胎发育及胚后发育中的相对表达丰度。研究结果显示,mi R-222在肌肉相关的组织中表达较高,特别是在成年翘嘴鳜的白肌中表达最高;胚胎发育阶段结果显示,mi R-222在胚胎发育的2细胞期就有表达,而表达量在心动期达到最高。不同组织及不同发育阶段的差异性表达结果表明,mi R-222很可能参与调控鳜鱼肌肉的生长发育。为研究合成代谢过程中mi R-222在肌肉生长调控中的表达规律,通过对翘嘴鳜幼鱼在饥饿一周后饱食一餐的实验处理下,利用实时荧光定量的方法测定mi R-222在骨骼肌中的相对表达变化。结果显示,mi R-222的表达量在恢复喂食后的1h显著上升（P〈0.05）,表明mi R-222很可能是调节鱼类骨骼肌生长过程中,参与快速应答信号系统的一类mi RNA。研究为mi R-222在鱼类发育中的调控作用提供一些理论依据。

英文摘要：

The mandarin fish （Siniperca chuatsi Basilewsky） is one of the most commercially important car-nivorous fish species in aquaculture. Increasing evidences have suggested that microRNAs （miRNAs） have emerged as key regulators of skeletal muscle growth, but little is known about miRNA expression profiles relate to skeletal muscle growth in teleost. The miR-222 has been reported as the key regulator during muscle differentiation. To investigate the expression pattern of miR-222 in different tissues and developmental stages in Siniperca chuatsi, the expression of miR-222 was detected by RT-qPCR. In the present study, we found that the miR-222 was highly expressed in muscle-related tissues, especially in fast （white） muscle of adult mandarin fish. RT-qPCR analysis revealed that the miR-222 was first detected in two cell stage em-bryos and highly expressed at the heart beating stage. The differential expression of miR-222 in different tissues and developmental stages indicated that miR-222 might involve in regulating muscle growth and de-velopment. We investigated the expression changes of the mature miR-222 abundance following a single sa-tiating meal in mandarin fish juveniles after fasting for a week. MiR-222 expression was sharply up-regulated within 1h after refeeding and might be desired candidate miRNA involved in a fast-response signaling system that regulates fish skeletal muscle growth. This finding would provide a novel insight in the role of miR-222 in teleost development.