中文摘要：

目的观察兔眼准分子激光原位角膜磨镶术（LASIK）及准分子激光上皮瓣下角膜磨镶术（Epi- LASIK）术后局部点用神经生长因子（NGF）后角膜组织中酪氨酸激酶受体A（TrkA）的表达情况，探索术后干眼症的防治方法。方法将30只新西兰纯种兔，抽取15只30眼接受LASIK手术，余接受Epi- LASIK手术。术后每只兔子一眼滴NGF滴眼液，为实验眼，对侧眼滴0.9%氯化钠溶液，为对照眼。1周后进行免疫组化分析，检测角膜上皮细胞层以及角膜基质层中TrkA的表达情况。结果观察TrKA的表达，接受LASIK或Epi- LASIK手术后，滴用NGF滴眼液的眼均较对照眼强。LASIK组以及Epi- LASIK组滴用NGF眼两者比较TrkA的表达无统计学差异。结论滴用NGF的兔眼，其LASIK或Epi- LASIK术后TrkA受体表达均较高，可能有助于防治近视激光术后干眼症。

英文摘要：

Objective To investigate the effect of nerve growth factor （NGF） on the expression of TrkA in cornea after LASIK or Epi- LASIK. Methods Sixty eyes of 30 New Zealand white rabbits underwent LASIK or Epi- LASIK procedures with 30 eyes in each group. One eye, randomly selected in each rabbit was treated with topical NGF for 1 week, while the contralateral eye was treated with normal saline as control. The expression of NGF receptor （TrkA） on cornea was detected by histoimmuno-chemistry. Results The expression of TrkA on the cornea treated with the topical NGF after LASIK or Epi- LASIK was stronger than that of control group. There was no difference in expression of TrkA between LASIK and Epi- LASIK groups. Conclusion Topical treatment of NGF can up- regulate the expression of TrkA in the cornea of rabbits after LASIK or Epi- LASIK.