中文摘要：

建立了超高效液相-电喷雾质谱（UPLC-ESIMS）法同时测定沙棘黄酮中槲皮素、杨梅素、芦丁、异鼠李素、山奈酚5种主要成分的定量测定方法。样品提取物用含内标的DMSO溶液溶解后过滤,滤液经色谱分离后,采用ESI-MS/MS在负离子多反应监测（MRM）模式下进行检测。该方法的相关系数R^2均大于0.999。最低检出限及定量下限分别为：槲皮素156.3ng/mL、312.5ng/mL,杨梅素7.8ng/mL、15.6ng/mL,芦丁9.8ng/mL、19.5ng/mL,异鼠李素3.9ng/mL、7.8ng/mL,山奈酚15.6ng/mL、62.5ng/mL。测定方法的日内精密度为：0.52～3.80;日间精密度为：2.61～4.04。5种成分的平均回收率为93.3%～104.9%。沙棘黄酮的5种成分含量分别为：槲皮素1.35mg/g、杨梅素0.25mg/g、芦丁1.74mg/g、异鼠李素0.96mg/g、山奈酚0.93mg/g。本文所用方法灵敏度高、操作简单高效,适用于沙棘果实中黄酮成分的定量分析。并对今后其它天然产物成分的定性定量分析奠定了坚实的基础。

英文摘要：

Establishment of the method of ultra high performance liquid chromatography electrospray ionization mass spectrometry（ UPLC- ESIMS） for the simultaneous quantitative determination of quercetin,myricetin,rutin,isorhamnetin,and kaempferol components in the seabuckthorn flavone. The samples were filtered by the DMSO solution containing the internal standard substance. After chromatographic separation of the filtrate,it was quantitatively detected by using ESI- MS / MS under the negative ion multiple reaction monitoring（ MRM） detection mode. The correlation coefficient（ R^2） of the method was more than 0. 999. The LLOD and LLOQ were shown as following respectively. Quercetin 156. 3ng / mL,312. 5ng / mL,myricetin7. 8ng / mL, 15. 6ng / mL, rutin 9. 8ng / mL, 19. 5ng / mL, isorhamnetin 3. 9ng / mL, 7. 8ng / mL, kaempferol 15. 6ng / mL,62. 5ng / mL. the intra- day and inter- day RSDis 0. 52- 3. 80 and 2. 61- 4. 04 respectively.The average recovery of the five constituents is 93. 3%- 104. 9%. The concentration of the five components of seabuckthorn flavone was shown as following,quercetin 1. 35 mg / g,myricetin 0. 25 mg / g,rutin 1. 74 mg / g,isorhamnetin 0. 96 mg / g,and kaempferol 0. 93 mg / g. the method used in this paper is highly sensitive,easy and efficient. It is suitable for the quantitative analysis of the flavonoids in seabuckthorn berries. The method has layed a solid foundation for future qualitative and quantitative analysis of other natural components.