中文摘要：

量的 PCR (RT-qPCR ) 与 Saccharopolyspora spinosa 的出版染色体信息相结合的反向的抄写能关于 S 允许复杂研究。spinosa 包括学习 spinosyn 生合成的规定，为工程发现新目标基因，并且发现并且利用另外的 macrolide 第二等的代谢物。研究证明了适当内部控制被需要在抄写层次使目标基因正常化。然而，许多研究证明了没有单个参考基因为在所有试验性的条件下面的所有种类是通用的。因此，三不同 S 的八候选人参考基因。在二种不同文化的 spinosa 种类被学习发现合适的参考基因。这些候选人基因的扩大周期的数字被 BestKeeper， NormFinder 和 geNorm 计算。结果显示为正规化的最合适的参考基因在 S 的发酵期间。spinosa 是 16S rRNA 和 rbL13。

英文摘要：

Reverse transcription quantitative PCR （RT-qPCR） combined with the published genome information of Saccharopolyspora spinosa can allow sophisticated studies about S. spinosa, including Studying the regulation of spinosyn biosynthesis, finding new target genes for engineering, and discovering and exploiting other macrolide secondary metabolites. Studies have demonstrated that appropriate internal control is needed to normalize target genes at transcription levels. However, many studies have shown that no single reference gene is universal for all strains under all experimental conditions. Thus, eight candidate reference genes of three different S. spinosa strains in two different cultures were studied to find suitable reference gene（sl. The number of amplification cycles of these candidate genes was calculated by BestKeeper, NormFinder and geNorm. The results indicated that the most suitable reference genes for normalization during the fermentation of S. spinosa were 16S rRNA and rbL13.