中文摘要：

目的研究大鼠骨髓间充质干细胞（MSC）在急性胰腺炎后肠黏膜损伤修复中的作用。方法从雄性SD大鼠股骨骨髓腔获取并培养MSC。20只雌性SD大鼠分成3组：急性重症胰腺炎（SAP）组（n=6）和MSC治疗组（n=8），均分2次以腹腔内注射L-精氨酸（2g／kg）造模，成模12h后，MSC治疗组予尾静脉注射MSC5×10^6个／只，连续3d，SAP组予尾静脉注射同体积0．9％NaCl溶液；正常对照组（n=6）仅腹腔注射0．9％NaCl溶液，未做任何处理。所有大鼠于造模72h后处死，取回肠组织HE染色行病理评分，RT-PCR方法检测回肠和胰腺组织中肿瘤坏死因子（TNF）-amRNA和白细胞介素（IL）-18mRNA表达水平，PCR检测胰腺和肠道组织的Y染色体性别决定基因Sry。结果SAP组和MSC治疗组胰腺中TNF—amRNA和IL-1βmRNA的相对表达量均．较正常对照组明显升高（7．22±1．99，3．46±1．75比1．32±1．04；2．71±0．56，1．92±0．28比0．61±0．45），差异有统计学意义（F=18．375，F=22．701；P〈0．05）；MSC治疗组中二者的相对表达量较SAP组明显下降，差异有统计学意义（P〈0．05）。SAP组和MSC治疗组肠道中TNF—amRNA和IL-1βmRNA的相对表达量均较正常对照组明显升高（3．93±1．08，2．13±0．53比0．68±0．42；2．44±1．54，1．02±0．44比0．60±0．14），差异有统计学意义（F=21．772，F=6．132；P〈0．05）；MSC治疗组中二者的相对表达量较SAP组明显下降，差异有统计学意义（P〈0．05）。病理评分显示：与SAP组相比，MSC治疗组大鼠肠黏膜病理损伤减轻（3．83±0．28比2．83±0．56），且差异有统计学意义（F=12．013，P〈0．05）。MSC治疗组中胰腺及肠道组织中均可检测到Sry基因表达。结论异体MSC移植抑制急性胰腺炎后促炎因子水平，减轻肠黏膜损伤，可能参与肠道组织的修复。

英文摘要：

Objective To investigate the role of bone marrow mesenchymal stem cells （MSCs） transplantation in repairing injured intestinal mucosa of acute pancreatitis. Methods MSCs were harvested and cultured from femurs of male SD rats. Twenty female SD rats were divided into three groups, and serve acute pancratitis （SAP） model was induced by intraperitoneal injection of L-arginine （2 g/kg） twice. Twelve hours after SAP model established, MSC transplantation group （n=8） were injected MSCs （5 × 10^6 celt/rat） through tail vein for three days, and SAP group （n= 6） were injected the same volume of saline through tail vein as control. Control group （n= 6） were only injected the same volume of saline without any treatment. All the rats were sacrificed at 72 hours after model established. The small intestinal tissues were taken for HE staining and pathological score, the TNF- a mRNA and IL-1β mRNA expression level in small intestine and pancreas were tested by RT-PCR. Y chromo-some （Sty） gene in pancreatic and intestinal tissue was examined by polymerase chain reaction （PCR）. Results The relative expression quantity of TNF-a mRNA and IL-1β mRNA in pancreas was significant higher in SAP group and MSC transplantation group than in control group （7.22 ± 1.99, 3.46±1.75 vs 1.32±1.04; 2. 71±0.56,1.92±0.28 vs 0. 61±0.45）, the difference was statistically significant （F= 18. 375, F= 22. 701; P〈0.05）. Compared with SAP group, the expression quantity of TNF-a mRNA and IL-1β mRNA in pancreas was significantly decreased in MSC transplantation group, the difference was statistically significant （P〈0.05）. The relative expression quantity of TNF- a mRNA and IL-1β mRNA in small intestine was significantly higher in SAP group and MSC transplantation group than in control group （3. 93±1.08,2. 13±0.53 vs 0. 68±0.42; 2. 44±1.54, 1. 02±0.44 vs 0. 60±0.14）, the difference was statistically significant （F=21. 772, F=6. 132; P〈 0.05）. The expression of TNF-a mRNA and IL-1