中文摘要：

粒细胞集落刺激因子（G—CSF）能够将骨髓干细胞动员到缺血组织参与组织修复，因而具有治疗心肌梗死的潜力．但是目前国际上对该疗法的效果尚存在争议．为了研究心肌梗死急性期内注射G—CSF对心肌纤维化及心脏功能的影响，将SD大鼠冠状动脉左前降支结扎3h后，皮下注射G—CSF（50μg／kg／d，共5d）或等量生理盐水．结果显示G—CSF注射5d后，外周血白细胞数量显著增加．3个月后，G—CSF注射显著增加了梗死范围和左心室胶原含量，损害了心脏功能．由以上可见，心肌梗死急性期内给予G—CSF治疗促进了心肌组织纤维化，并导致了心室扩张和心脏功能恶化．

英文摘要：

Controversy exists regarding the effects of granulocyte colony-stimulating factor （G-CSF） on post-infarction remodeling and functional recovery. In this study, a rat model of myocardial infarction （MI） was produced by permanent ligation of left anterior descending coronary artery. Three hours after MI, rats were randomized to receive subcutaneous G-CSF （50μg/kg/d for 5 consecutive days, n= 16） or saline （n= 10）. G-CSF injection significantly increased circulating white blood cells 5 days after MI. At 3 months, echocardiographic and hemodynamic examination revealed that the G-CSF treatment enhanced left ventricular （LV） enlargement and dysfunction. Histological analysis showed that the extent of myocardial fibrosis and infarct size were larger in the G-CSF group than in saline group. In conclusion, subcu- taneous injection of G-CSF immediately after acute myocardial infarction facilitated ventricular remodeling and deteriorated cardiac function in a rat model.