中文摘要：

目的：测定不同时期采收的香青兰药材中黄酮类成分（木犀草素-7-O-β-D-葡萄糖醛酸苷、芹菜素-7-O-β-D-葡萄糖醛酸苷、香叶木素-7-O-β-D-葡萄糖醛酸苷和田蓟苷）和酚酸类成分（迷迭香酸）的含量,确定最佳采收期。方法：采用PurospherSTAR LP RP-18 endcapped（4.6 mm×250 mm,5μm）色谱柱,以乙腈（A）-0.5%甲酸水溶液（B）为流动相,梯度洗脱（0-30 min,17% A;30-60 min,17% A→28% A;60-75 min,28% A）,流速1.0 mL·min-（-1）,检测波长330 nm,柱温35℃。结果：木犀草素-7-O-β-D-葡萄糖醛酸苷、芹菜素-7-O-β-D-葡萄糖醛酸苷、迷迭香酸、香叶木素-7-O-β-D-葡萄糖醛酸苷、田蓟苷5种化学成分的分离效果良好,质量浓度在相应的范围内与峰面积有良好的线性关系,相关系数均大于0.999 5;平均加样回收率（n=6）为98.4%-99.5%（RSD〈1.9%,n=6）。该方法应用于研究新疆吉木萨尔县产区不同采收期对香青兰药材中5种化学成分含量的影响,本试验在6月13日（种植后213 d）至8月6日（种植后270 d）内设置了8个采收期,发现木犀草素-7-O-β-D-葡萄糖醛酸苷等上述5种化学成分的含量与采收期（种植后天数）均呈二次或三次函数关系,随着采收期的推迟,含量先增后减。结论：该含量测定方法操作简单,重复性好,为确定香青兰药材适宜采收期提供依据。香青兰药材中木犀草素-7-O-β-D-葡萄糖醛酸苷等5种化学成分含量随采收期不同而异,其总量最大值出现在7月中上旬。

英文摘要：

Objective： To simultaneously determine the contents of luteolin-7-O-β-D-glucuronide, apigenin-7-O-β-D--glueuronide, diosmetin-7-O-β-D--glucuronide, and tilianin （ flavonoids ） and rosmarinincacid （ phenyl phenyl propanoids ） of Dracocephalum moldavica L. , so as to find out the best harvest time. Methods： HPLC was performed on a Purospher STAR LP RP-18 endcapped column （4.6 mm×250 mm,5μm ） at 35 ℃ with acetonitrile （ A ） -0.5% formic acid aqueous solution （ B ） as the mobile phase by gradient elution （0-30 min, 17%A; 30-60 min, 17%A → 28% A; 60-75 min, 28% A ）. The detection wavelength was 330 nm, and the flow rate was 1 mL · min^-1. Results： The baseline separation of these 5 components was achieved. The calibration curve was linear （ r 〉〉- 0. 999 5 ） over the tested ranges. The average recovery was 98.4%-99.5% （ RSD〈1. 9%, n=6 ）. The method has been successfully applied to the analysis of Dracocephalum moldavica in different harvest periods. In this paper, single-factor randomized block design was applied to study the 5 components content of Dracocephalum moldavica on eight collecting stages. For the Dracocephalum moldavica L. collected from June 13 to August 6, the contents of 5 ingredients increased primarily, and then decreased. Conclusion： The established method is proved to be simple and repeatable, which can provides the basis for the suitable harvest time of Dracocephalum moldavica . The contents of the 5 components are different in different harvest time. The maximum contents appear in the early of July.