通过病毒RNA抽提、RT-PCR、克隆,试验一次性获得野鸡源禽流感病毒A/Pheasant/Guangdong/GZI/2003(H9N2)NA全基因.序列分析及联机检索表明,该病毒的NA基因由1458bp组成,其中开放阅读框(ORF)l401bp编码466个氨基酸;ORF与A/Chicken/Guangdong/11/97同源性最高,达98%;与A/Chicken/Guangdong/11/97的NA亲缘性最近.
Based on the reported sequences of NA gene of avian influenza virus (AIV) H9N2 subtype, a pair of primers was designed for the amplification of NA gene from a strain of AIV H9N2 subtype isolated from a pheasant (A/Pheasant/Guangdong/GZI/2003(H9N2) by RT-PCR. Sequence analysis indicated that the NA gene was 1458 bp and the open reading frame (ORF) was 1401 bp in length, encoding for 466 amino acids, sharing more than 98% identities with A/Chicken/Guangdong/11/97(H9N2).