中文摘要：

鱼精朊是首先在精子发现的一组高度基本的蛋白质允许在基因抄写的下面规定的比 histones 和愿望结果的 DNA 的更稠密的包装[1 ] 。Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV ) 编码 P6.9，这很好被认出，通过绑定形成病毒的 subnucleosome 到病毒的染色体的像鱼精朊的蛋白质[29 ] 。以前的研究证明 P6.9 为病毒的 nucleocapsid 汇编是必要的，当它没在病毒的染色体复制上有影响时[31 ] 。在现在的学习，在病毒的基因抄写规定的 P6.9 的角色被描绘。与鱼精朊或另外的像鱼精朊的蛋白质相对照通常下面调整基因抄写， P6.9 出现到起来调整在 1224 小时柱子感染(hpi ) 的病毒的基因抄写，而它为病毒的基因抄写的基础水平是非本质的。荧光显微镜学表明有 DNA 的 P6.9s 合作本地化时间地并且空间地在病毒的基因抄写上与 P6.9s 影响被同步，显示 P6.9-DNA 协会贡献抄写规定。染色质分别试金进一步在 24 hpi 在一样的 transcriptionally 活跃的染色质部分揭示 P6.9 和主人 RNA 聚合酶 II 的意外共存，它可以可能在迟了的感染阶段贡献病毒的基因抄写起来规定。

英文摘要：

Protamines are a group of highly basic proteins first discovered in spermatozoon that allow for denser packaging of DNA than histones and will result in down-regulation of gene transcription~l~. It is well recognized that the Autographa californica multicapsid nucleopolyhedrovirus （AcMNPV） encodes P6.9, a protamine-like protein that forms the viral subnucleosome through binding to the viral genome[29]. Previous research demonstrates that P6.9 is essential for viral nucleocapsid assembly, while it has no influence on viral genome replication1311. In the present study, the role of P6.9 in viral gene transcription regulation is characterized. In contrast to protamines or other protamine-like proteins that usually down-regulate gene transcription, P6.9 appears to up-regulate viral gene transcription at 12-24 hours post infection （hpi）, whereas it is non-essential for the basal level of viral gene transcription. Fluorescence microscopy reveals the P6.9＇s co-localization with DNA is temporally and spatially synchronized with P6.9＇s impact on viral gene transcription, indicating the P6.9-DNA association contributes to transcription regulation. Chromatin fractionation assay further reveals an unexpected co-existence of P6.9 and host RNA polymerase II in the same transcriptionally active chromatin fraction at 24 hpi, which may probably contribute to viral gene transcription up-regulation in the late infection phase.