目的:探讨太空环境对甘草生理生化诱变作用分析。方法: 利用返回式卫星搭载甘草,18 d后返回地球(飞行回收舱平均辐射剂量为0.102 m·d^-1,飞行远地点距地球350 km,重力为1×10^-6m·s^-2),搭载种子返回地面后与对照组同时种植于实验田中,对太空搭载后甘草的过氧化氢酶(CAT),超氧化物歧化酶(SOD)的活性,甘草的蛋白含量以及蛋白电泳进行了考察。结果: 甘草经过卫星搭载后,甘草CAT,SOD酶活性均有不同程度提高,差异明显(P〈0.05),而且鄂托克前旗比杭锦旗酶活性均高。甘草经过卫星搭载后,甘草蛋白含量有一定程度提高,差异明显(P〈0.05),同时甘草蛋白电泳也产生了差异,产生了微弱的新的条带。结论: 太空环境对甘草蛋白类产生一定影响,这些变化对于后期选育甘草优良种质奠定基础。
Objective: To investigate the space environment on the role of licorice mutagenesis analysis of proteins. Method: Licorice (Glycyrrhiza uralensis) seeds were carried by a recover-able satellite for 18 days(the average radiation dose in the flight recovery module was 0.102 m·d^-1, the orbit semidiameter 350 km, gravity 10^-6). After returned to earth, The satellite-flown seeds and the un-flown seeds (ground control) were planted in the fields of experimental farm. The leaves of each group were used for studying the effects of space flight on CAT, SOD activity, the protein content and electrophoresis. Result: After the satellite, CAT, SOD activity in liquorice were increase in varying degrees, the difference obviously (P〈0.05), two types of enzyme activity of Ordos were higher than Hangjinqi. The protein content of Licorice increased in a certain extent, the difference obviously (P〈0.05), while protein electrophoresis also had differences, resulted in weak new bands. Conclusion: These results indicated that spaceflight induce the protein of Licorice, these changes maybe used as a tool for accelerating the progress in G. uralensis breeding.