中文摘要：

目的：静电纺是一种使带电荷的聚合物溶液或熔体在静电场中射流来制备聚合物纳米级纤维的加工方法,采用此种技术制备壳聚糖/胶原蛋白复合纳米纤维,并观察其细胞相容性。方法：实验于2006-11/2007-10在东华大学化学化工与生物工程学院生物材料与组织工程实验室完成。①支架材料制备：以六氟异丙醇/三氟乙酸为溶剂体系,采用静电纺制备复合纳米纤维,其中壳聚糖/胶原蛋白的质量比分别为100∶0,80∶20,50∶50,20∶80与0∶100。②细胞相容性观察：体外接种猪髋动脉内皮细胞,苏木精-伊红染色法观察细胞形态,MTT法检测细胞黏附和增殖情况。结果：猪髋动脉内皮细胞在壳聚糖/胶原蛋白复合纤维表面贴附牢固,外形饱满,多呈长梭形,具有良好的生长形态;MTT法结果显示纳米纤维能够有效地促进内皮细胞在材料表面的黏附和增殖,质量比为20∶80材料组细胞黏附、增殖能力最强,其次为50∶50组。结论：静电纺壳聚糖/胶原蛋白复合纳米纤维具有良好的细胞相容性,可望成为一种新型的组织工程支架材料。

英文摘要：

AIM： Electrospunning is a process that the charged polymer solution or melt jet efflux in the electrostatic field to prepare the polymer nanofibers. This study aimed to prepare electrospun chitosan/collagen composite nanofibers and evaluate their cell biocompatibility. METHODS： The experiment was carried out in the Laboratory of Biomaterials and Tissue Engineering, College of Chemistry, Chemical Engineering and Biotechnology at Donghua University between November 2006 and October 2007. （1） Taking hexafluoroisopropanol/trifluoroacetic acid as solvent, the composite nanofibers were prepared by electrospunning according to the mass ratio of chitosan/collagen as 100：0, 80：20, 50：50, 20：80 and 0：100.（2）The composite nanofibers were inoculated with porcine iliac artery endothelial cells in vitro. The cell adhesion and proliferation as well as cell morphologies were evaluated by MTT method and HE stain, respectively. RESULTS： The porcine iliac artery endothelial cells adhered tightly on surface of electrospun chitosan/collagen composite nanofibers, exhibited plump and fusiform shape, as well as good growth. MTT results showed that nanofibers could obviously enhancc the adhesion and proliferation of endothelial cells on the surface of materials, and the cells had the best spreading and attachment on the composite nanofibers with a ratio of 20：80, then 50：50. CONCLUSION： The electrospun chitosan/collagen composite nanofibers hold great potential for tissue engineering application due to their good cell biocompatibility.