为探索除草剂阿特拉津对红耳龟胚胎发育及肝脏、肾脏组织学变化的影响,本试验模拟不同含量阿特拉津污染环境,设置对照组(蒸馏水)和0.005mg/cm2、0.025mg/cm2、0.125mg/cm23个试验组,在温度(30±0.5)℃、相对湿度为85%~90%的条件下孵化红耳龟受精卵,检测孵化率、孵化期及肝、肾脏组织结构变化指标。结果表明,0.005mg/cm2、0.025mg/cm2的阿特拉滓对孵化率和孵化期没有显著影响,0.125mg/cm2组孵化期显著延长(P〈0.01);试验处理组肝脏和肾脏有不同程度的损伤,0.005mg/cm2组主要表现增生性病变,0.025mg/cm2和0.125mg/cm2组损伤作用加重,主要表现为退行性病变。试验结果说明,阿特拉津长时间作用红耳龟受精卵可能导致胚胎发育迟缓,并导致肝脏和肾脏组织病变。
To investigate the effects of atrazine on embryonic development and histological changes in liver and kidney in red-eared turtle (Trachemys scripta elegans), the fertilized eggs of red-eared turtle were in- cubated in the water containing atrazine at a dose of 0. 005 mg/cm2 (low-dose group), 0. 025 mg/cm2 (mid- dle-dose group), 0. 125 mg/cm2 (high-does group) and distilled water (control group) at (30±0.5) ℃ un- der the relative humidity range of 85 to 90 percent in a laboratory. During the incubation, the hatching rate, incubation period and histological changes in liver and kidney were observed. The results showed that the incubation period was significantly prolonged in high-dose group whilst the incubation period and hatching rate were no significant effect in both low-dose and middle-dose groups. Also the damaged liver and kidney tissues had hyperplastic lesions in low-dose group and the degenerative changes in the middle- dose and high-dose groups. The findings illustrated that atrazine may lead to developmental delay in the red-eared turtle embryo and to histological lesions in the kidney and liver.