中文摘要：

目的：以ob／ob基因敲除肥胖小鼠为研究模型。探讨肥胖对小鼠肝脏炎症的影响以及ob／ob小鼠肝脏中与能量代谢相关转录因子：过氧化物酶体增殖物激活受体γ共激活因子-1α（peroxisome proliferator activated receptor γ coactivator--1α，PGC-1α）、过氧化物酶体增殖物激活受体α（peroxisome proliferator activatedreceptor α ，PPARα）、核呼吸因子1（nuclear respiratory factor1，NRF1）的基因表达特征。方法：选取以C57BL／6J为背景的雄性ob／ob肥胖小鼠（以下简称ob／ob小鼠）作为肥胖模型．同龄正常C57BL／6J雄性小鼠作为对照，2组小鼠各8只，于SPF级动物房饲养至5月龄，处死后取肝组织，石蜡包埋切片，HE染色观察组织形态；抗原F4／80免疫组化染色观察巨噬细胞浸润情况；RT—PCR检测各转录因子mRNA表达水平。结果：5月龄ob／ob小鼠体质量和肝脏质量高于对照组（t=9．66，P=O．000；t=9．98，P=-0．000）；石蜡切片HE染色结果显示ob／ob小鼠肝细胞胞浆内出现空泡状脂肪滴；F4／80免疫组化结果显示ob／ob小鼠肝脏组织巨噬细胞浸润明显；RT—PCR结果显示ob／ob小鼠肝脏组织转录因子PGC-1α、PPARα、NRFl的mRNA表达水平较对照组明显上调（t=3．02，P=0．009；f=5．64，P=0．000；t=2．93，P=0．011）。结论：ob／ob小鼠表现出显著的肥胖特征，肝细胞出现明显脂质沉积，肝组织炎性细胞浸润；上述改变可能与肝脏能量代谢相关转录因子PGC—1α．PPARα和NRF1的表达增加有关。

英文摘要：

Objective：To examine the mRNA expression characteristics of hepatic energy metabolism-related transcription factors： peroxisome proliferator activated receptor γ, coactivator- 1 α （PGC- lα）, peroxisome proliferator activated receptor ot （PPARα） and nuclear respiratory factor I（NRF1） in ob/ob mice. Methods：Male ob/ob obese mice on a C57BL/6J genetic background were used as obese model（n=8） and normal male C56BL/6J mice as control（n=8）. At the age of 5 months,liver samples were obtained after sacri- ficing. Liver morphologic analysis was performed by HE staining and macrohoages were stained by immunohistochmistry method us- ing F4/80 antibody. RT-PCR was performed to determine the mRNA expression levels of interested genes. Results： Both body weight and liver weight of the ob/ob mice were higher than those of control group（t=9.66,P=0.000;t=9.98,P=0.000）. In the ob/ob group, HE showed lipid accumulation. Immunohistochmistry staining for F4/80 results showed more severe inflammatory cell infiltration in ob/ ob group. Compared with those in control group,PGC-lα,PPARα,NRF1 mRNA expression levels increased significantly（t=3.02, P=0.009;t=5.64,P=0.000;t=2.93,P=-0.011）. Conclusion：The ob/ob mice are obese than normal controls. There are significant hepatic lipid accumulation and inflammatory cell infiltration in the livers of ob/ob mice. which may related with the increased expressions of PGC-lα,PPARα and NRF1.