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中文摘要：
为解决酶固定化过程中稳定性较差的问题,将单维交联酶策略引入青霉素酰化酶的固定化中,通过在介孔泡沫硅载体（MCFs）表面初步吸附作用将酶固定,再用对本醌对酶分子进行交联,从而制得单维交联酶聚体。实验结果显示：单维交联酶聚体（CLEA）的稳定性有明显提高,最适温度从游离酶的45℃上升到55℃,催化温度的适用范围更宽,在50℃下热处理25 h仍保持74.4%活力,在测试操作稳定性方面,连续操作15次,其酶活保持在61.9%。
英文摘要：
In order to improve the stability of penicillin acylase（PA）,the strategy of one-dimensional cross-linked enzyme aggregate（CLEA） was introduced to the immobilization process of PA.Mesocellular siliceous foams（MCFs） with large pore diameter were synthesized,and PA was coupled to MCFs through adsorption and covalent linkage by benzoquinine.The experimental results show that the stability of PA is greatly increased,and the optimal temperature of PA is increased from 45 ℃ to 55 ℃.After thermal treatment at 50 ℃ for 25 h,the activity of immobilized enzyme remains 74.4%,maintaining 61.9% activity after repeated use for 15 times.
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