中文摘要：

背景丙肝病毒( HCV )信封基因编码 glycoproteins E1 和 E2 展出产生不同 phenotypic 特点的可变性的高度；包括在受体绑定的改变，亲密关系和有免疫力的识别和 escape.This 学习试图阐明为到病毒的 persistence.Methods HCV 的 HCV 信封 glycoproteins 的进化模式的关系 quasispecies 在每二～六个月从尖锐地 感染HCV 的 subj 的一个队收集的标本被描绘

英文摘要：

Background Hepatitis C virus （HCV） envelope genes encoding glycoproteins E1 and E2 exhibits a high degree of variability that gives rise to differing phenotypic traits; including alterations in receptor-binding affinity and immune recognition and escape. This study aims to elucidate the relationship of the evolutionary patterns for HCV envelope glycoproteins to viral persistence. Methods HCV quasispecies were characterized in specimens collected every two to six months from a cohort of acutely HCV-infected subjects. We evaluated two individuals who spontaneously cleared viremia and three individuals with persistent viremia by cloning 33 1-kb amplicons that spanned E1 and the 5＇ half of E2; including hypervariable region 1 （HVR1）. To detect representative variants for sequencing thirty-three cloned cDNAs representing each specimen were assessed by a method that combined analysis of a single-stranded conformational polymorphism （SSCP） method and heteroduplex analysis （HDA）. For each patient, the rates of both synonymous and nonsynonymous substitutions for the El, HVR1 and E2 regions outside HVR1 were evaluated. The amino acid sequences and predicted antigenic profiles were analyzed. Results The genetic diversity within HVR1 was consistently higher than that in the E1 and E2 regions outside HVR1 in individuals with persistent viremia, but did not change markedly over time in those with clearance of viremia. For individuals with persistent viremia, the rate of nonsynonymous substitutions within the HVR1 region predominated and gradually increased, compared to that in the E1 and E2 regions outside HVR1. By contrast, the rates of both nonsynonymous and synonymous substitutions for the E1 and E2 regions, including HVR1, were consistently lower in individuals with clearance of viremia. HVR1 had a higher antigenic variable and lower positive charge in subjects with persistent viremia. All cysteine residues and N-linked glycosylation sites, some of which were known to play a major role in protein foldin