中文摘要：

目的探究骨桥蛋白（Osteoponfin）和E一钙粘素（E—cadherin）及其联合检测对放疗抵抗及预后的意义。方法回顾性分析132例初始放射治疗的局部进展期宫颈鳞癌（LACSCC）患者，将无进展生存期（PFS）〈36个月的患者（n=47）归为放疗抵抗组，PFS≥36个月的患者（n=85）归为放疗敏感组，使用治疗前石蜡包埋组织，采用免疫组织化学的方法检测Osteoponfn及E—cadhefin表达情况。结果放疗抵抗组和放疗敏感组肿瘤组织中，Osteoponfin高表达率分别占87．2％和30．6％，组间差异具有统计学意义（P=0．000）；E—cadherin低表达率分别占72．3％和57．6％，组间差异不具有统计学意义（P=0．094）；Osteopontin高表达且E—cadherin低表达表型分别占72．3％和18．8‰组间差异具有统计学意义（P=0．000）。多因素Cox回归分析显示LACSCC的不良预测因素包括Osteopontin表达，Osteopontin高表达且E-cadherin低表达和肿瘤大小。Osteopontin高表达和Osteopontin高表达同时E—cadherin低表达的患者无进展生存期更短。结论高表达Osteopontinhe和高表达Osteopontin同时低表达E—cadherin提示放疗抵抗及顸后不良，联合检测Osteoponfin和E—cadhefin有望成为预测放疗疗效和患者预后的指标。

英文摘要：

[ Objective ] To investigate the association of expression of osteopontin and E-cadherin with radiosen- sitivity in patients with locally advanced cervical squamous cell carcinoma （LACSCC）. [ Methods ] We retrospective- ly reviewed 132 female patients who received primary radiation therapy to treat LACSCC at FIGO stages IBIVA. Forty-seven patients with progression-free survival （PFS） less than 36 months were defined to be radiation-resistant. Eighty-five patients with PFS no less than 36 months were regarded as radiation-sensitive. In pretreated paraffin- embedded tissues, we evaluated osteopontin and E-eadherin expression using immunohistochemistry. [Results] High expression of osteopontin in radiation-resistant and radiation-sensitive patients was 87.2% and 30.6%, respec- tively, and this difference was statistically significant （P = 0.000）. Low expression of E-cadherin in radiation-resis- tant and radiation-sensitive patients was 72.3% and 57.6%, respectively, and this difference was not statistically sig- nificant （P = 0.094）. High osteopontin and low E-cadherin expression in radiation-resistant group and radiation- sensitive group were 72.3% and 18.8% respectively, and the difference was statistically significant （P = 0.000）. The 5-year PFS rates were 38.1% and 81.8% respectively in patients with high osteopontin but low E-eadherin expres- sion （n = 50） and the rest （n = 82） （P = 0.000）. High osteopontin expression alone, high osteopontin and low E-cad-herin expression, tumor diameters [Hazard ratios （95%CI） were 2.847 （1.048, 7.730）, P = 0.040; 2.571 （1.121, 5.894）, P = 0.026 and 2.635 （1.462, 4.750）, P = 0.001 respectively] were independent poor prognostic factors for patients with LACSCC in multivariate Cox regression analysis. [ Conclusions ] Patients with high osteopontin and low E-cadherin expression showed greater resistance to radiotherapy and a shorter PFS than the rest. In particular, high osteopontin and low E-cadherin expression can be considered as