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中文摘要：
目的 探讨绿原酸（CGA）对转化生长因子（TGF）-β1诱导肝星形细胞（HSC）分泌肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6的影响,以及P38MAPK、核转录因子（NF）-κB信号通路调控机制。方法 不同质量浓度CGA作用于正常和转化生长因子（TGF）-β1诱导肝星形细胞,确定合适药物质量浓度;指数生长期的细胞随机分为正常HSC组、正常HSC＋CGA组：细胞培养48 h后,换含终质量浓度0、50、100 mg·L-1CGA的DMEM培养液培养24 h。HSC（TGF-β1）组、HSC（TGF-β1＋CGA）组,细胞培养24 h后,用含终质量浓度为10μg·L-1TGF-β1的DMEM培养液刺激24 h,换含终质量浓度0、50、100 mg·L-1CGA的DMEM培养液培养24 h。免疫细胞化学法检测细胞中α-SMA蛋白的表达,Western-blot检测p-P38、P65表达,实时定量聚合酶链反应（PCR）检测TNF-α、IL-6 mRNA的表达,ELISA法检测上清液中TNF-α、IL-6的含量。结果 确定合适CGA质量浓度为50、100 mg·L-1,50及100 mg·L-1的CGA对正常HSC无影响（P〉0.05）;10μg·L-1TGF-β1诱导后,细胞中α-SMA、p-P38、P65、TNF-α、IL-6的表达显著增加（P〈0.01）,50及100 mg·L-1的CGA处理TGF-β1诱导HSC细胞,α-SMA、p-P38、P65、TNF-α、IL-6的表达显著降低（P〈0.05,P〈0.01）。结论 CGA能抑制TGF-β1诱导HSC的增殖,调控P38 MAPK、NF-κB信号通路,调节TNF-α,IL-6炎性因子的分泌,抑制肝纤维化的发生。
英文摘要：
OBJECTIVE To observe the effect of chlorogenic acid on the secretion of inflammatory cytokines and regulation mechanism of P38MAPK, NF-KB signaling pathway in human hepatic stellate cells induced by TGF-β1. METHODS Different concentrations of CGA worked in normal and activated hepatic stellate cells to make sure the appropriate drug concentration. The exponential growth phase cells were randomly divided into normal HSC group, normal HSC ＋ CGA group, after cultured 48 h, the cells were cultured with 0,50,100 mg·L^-1 CGA for 24 h ; HSC （ TGF-β1 ） group, HSC （ TGFA31 ＋ CGA） group ： after 24 h, the cells were induced by 10 μg·L^-1 TGF-β1 for 24 h, and then cultured with 0, 50, 100 mg·L^-1 CGA for 24 h. The expression of α-SMA protein was detected by immunocytochemistry, the expression of p-P38, P65 protein was detected by Western- blot, the expression of TNF-a, IL-6 mRNA was detected by real time quantitative PCR, and the content of TNF-α, IL-6 in the supernatant was detected by ELISA method. RESULTS The appropriate concentrations of CGA were 50 and 100 mg·L^-1, these concentration has no effect on normal HSC （ P 〉 0.05 ） ; after stimulation by TGF-β1, the expression of α-SMA, p-P38, P65, TNF-α, IL-6 was increased（P 〈0.01 ） , when activated HSC cells were treated with 50 and 100mg·L^-1 CGA, the expression of α-SMA, p-P38, P65, TNF-α, IL-6 was decreased（P 〈 0.05, P 〈 0.01 ）. CONCLUSION CGA can inhibit the proliferation of activated HSC, regulate the secretion of inflammatory factors such as TNF-α, IL-6 by P38MAPK and NF-κB signaling pathway, inhibit the occurrence of liver fibrosis.
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