中文摘要：

目的优化超声波辅助乙醇提取藤茶总黄酮的工艺条件,提高藤茶总黄酮的提取效率。方法在单因素的基础上,应用Box-Behnken中心组合法进行4因素3水平的试验设计,研究乙醇浓度、超声波处理温度、超声波处理时间、液料比对总黄酮得率的影响。结果藤茶总黄酮的最优超声波辅助乙醇提取条件为:乙醇浓度67%(V:V),超声波处理温度58℃,超声波处理时间28 min,液料比22.5:1(V:m)。模型预测结果显示藤茶总黄酮得率最大为38.45%,经过验证,实际得率为38.74%,接近预测值,说明预测值和真实值之间有很好的拟合性,用响应面优化法得到的结果科学有效,有较高的参考价值。结论采用超声波辅助提取法,总黄酮得率高,用时少,具有很好的应用前景。

英文摘要：

Objective To optimize the extraction conditions by ultrasonic-assisted ethanol method to extract total flavonoids from Ampelopsis grossedentata in order to enhance the total flavonoids yield. Methods Based on single-factor tests, a 4-factor and 3-level center composite experimental design of Box-Behnken was adopted to investigate the influence of 4 main factors including alcohol concentration, ultrasonic treatment temperature, ultrasonic treatment time and liquid-solid ratio. Results The optimum conditions of ultrasonic-assisted extraction technology of total flavonoids from Ampelopsis grossedentata were as follows: alcohol concentration was 67% (V:V), ultrasonic treatment temperature was 58 ℃, ultrasonic treatment time was 28 min and liquid-solid ratio was 22.5:1 (V:m). The maximum predicted yield for total flavonoids from Ampelopsis grossedentata was 38.45% and the actual yield was 38.74%, which was near to the predicted yield, showing that there is a high fitting degree between predicted and real numerical values. The experimental results are valuable, because the response surface methodology is scientific. Conclusion The application of ultrasonic-assisted extraction not only result in a high yield of total flavonoids, but also greatly shorten processing time. Thus, the ultrasonic-assisted extraction is very promising in this area.