针对四川花生主产区酸性紫色土缺氮、磷、钼的现状,并依据“高效花生根瘤菌+Mo”显著的增产效果和丛枝菌根(AM)真菌高效吸磷特性,分别将R(高效花生根瘤菌)+Mo、AM菌剂(Clomusmosseae)、AM+R+Mo对盆栽花生进行处理。试验结果如下:(1)单接种G.mosseae能显著提高土著根瘤菌的结瘤能力,极显著提高盛花期植株含磷量。植株盛花期干重、叶绿素、总氮、总钾量高于单接种花生根瘤菌Spr4-5(R)和CK,且与CK达到显著或极显著水平。单接种根瘤菌Spr4-5比单接种G.mosseae增产10.1%,差异不显著;分别比CK增产52.5%、38.5%,差异均达极显著水平。Spr4-5和G.mosseae均是高效菌株。(2)Spr4-5和G.mosseae双接种比单接种Spr4-5或G.mosseae分别增产3.8%、14.3%,差异不显著;比CK增产58.3%,达极显著水平。(3)“AM+Spr 4—5+钼酸铵”菌剂处理比等钼量的“Spr4-5+钼酸铵”菌剂的产量低,且达到极显著水平;G.mosseae对钼敏感;与供试根瘤菌正协同效果最佳的钼酸铵浓度为2‰。
It is well known that N,P,Mo were deficient in acid purple soil in the main peanut growing region of Sichuan Province. And arbuscular mycorrhizal fungi can efficiently utilize phosphorus and inoculation of peanut with Bradyrhizobium Spr 4-5 and molybdate can increase yield significantly. We carried out a pot experiment with peanut grown in acid purple soil inoculated with peanut Bradyrhizobium and molybdate or/and AM fungus Glomus mosseae. Results showed that (1) single-inoculation with G. mosseae could significantly enhance indigenous strains nodulation ability, dry wt. ,chUophyll, total N, total K2O,total P2O5 content in plants compared with CK. The yield of peanut plants inoculated with single Spr 4-5 increased by 10.1% compared with G. mosseae,The yield increased by 52.5% ,38.5% with inoculating Spr 4-5 or G. mosseae respectively, compared with CK. Therefore, the tested strains Spr 4-5 and G. mosseae were both highly effective. (2) The yield increased significantly by 3.8% ,14.3% 58.3% with co-inoculating Spr 4 - 5 + G. mosseae, compared with Spr 4-5, G. mosseae, or CK, respectively. (3) The yield of peanut plants co-inoculated with G. mosseae + Spr 4 - + Ammonium molybdate decreased significantly compared with co-inoculated Spr 4-5 + Ammonium molybdate. The result indicated that the tested strain G. mosseae was sensitive to Mo. And the optimum ammonium molybdabte concentration for synergistic efficiency of Spr 4-5 with Mo was 2‰.