中文摘要：

背景细胞色素 P450 2E1 (CYP2E1 ) 在象 N-nitrosoamines 和另外的低相对的分子的团那样的 precarcinogens 的新陈代谢的激活有一个重要角色，有机化合物。这研究检验了 CYP2E1 Rsal 和 Dral 多型性是否与危险性被联系到在遗传型和有最新诊断的、未经治疗的食道的有鳞的房间癌和 79healthy 控制的 77 个病人在年龄匹配的 CYP2E1 mRNA.Methods 的表示层次之间的食道的有鳞的房间癌和关联，性和住处为控制学习被招募。在 5'-flanking 区域的 Rsal 多型性和在 CYP2E1 基因的第六 intron 的 Dral 多型性，能可能影响它的抄写，被聚合酶链反应限制碎片长度多型性( PCR-RFLP )在这研究决定， CYP2E1 的 mRNA 水平被量的即时反向的抄写 PCR.Results 测量 Rsal 或有食道的有鳞的房间癌的危险性的 CYP2E1 的 Dral 多型性的重要协会都没被表明(0.89-3.15， P=0.11；OR=1.11， 95% CI：0.59-2.09， P=0.74，分别地) 。用 SHEsis 软件，没有连接不平衡在 Rsal 和 Dral 多型性之间被检测(D'=0.528， r2=0.27 ) 。什么时候与 Dral 多型性，食道的有鳞的房间癌的在那带的 c2 等位基因之间的协会和 DD 遗传型和风险联合了 Rsal 多型性，被发现(OR=5.77， 95% CI：1.65-20.22 ) 。与正常控制，有 Rsal 多型性的 mRNA 层次， Dral 多型性，或任何东西相比，在情况中的联合遗传型没显示出这研究建议的统计 difference.Conclusions 那 carryingc2 等位基因和 DD 遗传型 conferreded 为食道的有鳞的房间癌的提高的风险。在遗传型 c1/c2， D/C，或联合等位基因和 mRNA 表示之间没有重要统计关系。

英文摘要：

Background Cytochrome P450 2E1 （CYP2E1） has an important role in the metabolic activation of precarcinogens such as N-nitrosoamines and other low relative molecular mass, organic compounds. This study examined whether CYP2E1 Rsal and Dral polymorphism are associated with susceptibility to esophageal squamous cell carcinoma and the correlation between the genotypes and expression levels of CYP2E1 mRNA. Methods Seventy-seven patients with newly diagnosed, untreated esophageal squamous cell carcinoma and 79 healthy controls matched in age, gender and residence were recruited for the control study. An Rsal polymorphism in the 5＇-flanking region and a Dral polymorphism in the sixth intron of the CYP2E1 gene, which could possibly affect its transcription, were determined in this study by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） and mRNA level of CYP2E1 was measured by quantitative real-time reverse transcription PCR. Results No significant association of Rsal or Dral polymorphism of CYP2E1 with susceptibility of esophageal squamous cell carcinoma were demonstrated （OR=1.67, 95%CI： 0.89-3.15, P=0.11; OR=1.11, 95%CI： 0.59-2.09, P=0.74, respectively）. With SHEsis software, no linkage disequilibrium was detected between Rsal and Dral polymorphism （D＇=0.528, r^2=0.27）. When combined Rsal polymorphism with Dral polymorphism, the association between that carrying c2 allele and DD genotype and the risk for esophageal squamous cell carcinoma were found （OR=5.77, 95%CI： 1.65-20.22）. Compared with the normal controls, the mRNA levels with Rsal polymorphism, Dral polymorphism, or any combined genotypes in cases showed no statistical difference. Conclusions This study suggests that carrying c2 allele and DD genotype conferreded an elevated risk for esophageal squamous cell carcinoma. There was no significant statistical relationship between the genotypes c1/c2, D/C, or the combined allele and mRNA expression.